-
Article
Open AccessDonor genetic burden for cerebrovascular risk and kidney transplant outcome
Kidney grafts from donors who died of stroke and related traits have worse outcomes relative to grafts from both living donors and those who died of other causes. We hypothesise that deceased donors, particula...
-
Article
Probing when dCas9 tolerates DNA mismatches
Sensing changes in ionic current as barcoded DNA translocates through solid-state nanopores allows the study of how nucleotide sequences alter the DNA-binding specificity of the catalytically inactive Cas9 rib...
-
Article
Open AccessMaternal educational attainment in pregnancy and epigenome-wide DNA methylation changes in the offspring from birth until adolescence
Maternal educational attainment (MEA) shapes offspring health through multiple potential pathways. Differential DNA methylation may provide a mechanistic understanding of these long-term associations. We aimed...
-
Article
Open AccessDevelopment of a proteomic signature associated with severe disease for patients with COVID-19 using data from 5 multicenter, randomized, controlled, and prospective studies
Significant progress has been made in preventing severe COVID-19 disease through the development of vaccines. However, we still lack a validated baseline predictive biologic signature for the development of mo...
-
Article
Open AccessGenome-wide association meta-analysis identifies risk loci for abdominal aortic aneurysm and highlights PCSK9 as a therapeutic target
Abdominal aortic aneurysm (AAA) is a common disease with substantial heritability. In this study, we performed a genome-wide association meta-analysis from 14 discovery cohorts and uncovered 141 independent as...
-
Article
Open AccessAnalysis of DNA methylation at birth and in childhood reveals changes associated with season of birth and latitude
Seasonal variations in environmental exposures at birth or during gestation are associated with numerous adult traits and health outcomes later in life. Whether DNA methylation (DNAm) plays a role in the molec...
-
Article
Proteomic analysis of diabetes genetic risk scores identifies complement C2 and neuropilin-2 as predictors of type 2 diabetes: the Atherosclerosis Risk in Communities (ARIC) Study
Genetic predisposition to type 2 diabetes is well-established, and genetic risk scores (GRS) have been developed that capture heritable liabilities for type 2 diabetes phenotypes. However, the proteins through...
-
Article
Associations of plasma omega-3 and omega-6 pufa levels with arterial elasticity: the multi-ethnic study of atherosclerosis
Literature examining the relationship of circulating omega-3 and omega-6 polyunsaturated fatty acids [n-3(ω-3) and n-6 (ω-6) PUFAs] and arterial elasticity in large cohort-based populations are lacking. We inv...
-
Article
Open AccessDNA methylation signature of chronic low-grade inflammation and its role in cardio-respiratory diseases
We performed a multi-ethnic Epigenome Wide Association study on 22,774 individuals to describe the DNA methylation signature of chronic low-grade inflammation as measured by C-Reactive protein (CRP). We find 1...
-
Article
Donor and recipient polygenic risk scores influence the risk of post-transplant diabetes
Post-transplant diabetes mellitus (PTDM) reduces allograft and recipient life span. Polygenic risk scores (PRSs) show robust association with greater risk of develo** type 2 diabetes (T2D). We examined the a...
-
Article
Open AccessDynamics of plasmid-mediated niche invasion, immunity to invasion, and pheromone-inducible conjugation in the murine gastrointestinal tract
Microbial communities provide protection to their hosts by resisting pathogenic invasion. Microbial residents of a host often exclude subsequent colonizers, but this protection is not well understood. The Enteroc...
-
Protocol
Evaluating Reliability of DNA Methylation Measurement
DNA methylation is a widely studied epigenetic phenomenon. Alterations in methylation patterns influence human phenotypes and risk of disease. The Illumina Infinium HumanMethylation450 (HM450) and MethylationE...
-
Protocol
An Ultracompact Real-Time Fluorescence Loop-Mediated Isothermal Amplification (LAMP) Analyzer
Low-cost access to the highly sensitive and specific detection of the pathogen in the field is a crucial attribute for the next generation point-of-care (POC) platforms. In this work, we developed a real-time ...
-
Protocol
Sample-to-Answer Microfluidic Nucleic Acid Testing (NAT) on Lab-on-a-Disc for Malaria Detection at Point of Need
One of the grand challenges for field-deployable NATs is related to the front end of the assays—nucleic acid extraction from raw samples. The ideal nucleic acid sample preparation should be simple, scalable, a...
-
Article
Open AccessAssociations between DNA methylation and BMI vary by metabolic health status: a potential link to disparate cardiovascular outcomes
Body mass index (BMI), a well-known risk factor for poor cardiovascular outcomes, is associated with differential DNA methylation (DNAm). Similarly, metabolic health has also been associated with changes in DN...
-
Article
Plasma omega-3 and saturated fatty acids are differentially related to pericardial adipose tissue volume across race/ethnicity: the Multi-ethnic Study of Atherosclerosis
Pericardial adipose tissue (PAT) is a cardiometabolic risk factor influenced by race/ethnicity, inflammation, and metabolic dysfunction. Omega-3 fatty acids (FAs) and saturated FAs (SFAs) are known to affect t...
-
Article
Open AccessPublisher Correction: A multi-ethnic epigenome-wide association study of leukocyte DNA methylation and blood lipids
-
Article
Open AccessA multi-ethnic epigenome-wide association study of leukocyte DNA methylation and blood lipids
Here we examine the association between DNA methylation in circulating leukocytes and blood lipids in a multi-ethnic sample of 16,265 subjects. We identify 148, 35, and 4 novel associations among Europeans, Af...
-
Article
Open AccessSystem dynamics modelling of urbanization under energy constraints in China
The rapid urbanization in China has been associated with a growing hunger for energy consumption and steadily-increasing CO2 emissions. In this paper, an integrated system dynamics model composed of four sub-mode...
-
Article
Open AccessLung Function and Gene Expression of Pathogen Recognition Pathway Receptors: the Cardia Lung Study
Activation of toll-like receptors (TLR1, TLR5, TLR6) and downstream markers (CCR1, MAPK14, ICAM1) leads to increased systemic inflammation. Our objective was to study the association between the gene expression l...
