46 Result(s)

Article

Molecular characterization and functional analysis of Glycine max sterol methyl transferase 2 genes involved in plant membrane sterol biosynthesis

Sterol C24 methyltransferase (SMT2) genes governing the pattern of phytosterols synthesized in higher plants have been studied in Glycine seedlings and wild-type and engineered Arabidopsis thaliana plants. The SM...

Anjanasree K. Neelakandan, Hanh T. M. Nguyen, Rajesh Kumar… in Plant Molecular Biology (2010)

Article

CYP51: A Major Drug Target in the Cytochrome P450 Superfamily

The cytochrome P540 (CYP) superfamily currently includes about 9,000 proteins forming more than 800 families. The enzymes catalyze monooxygenation of a vast array of compounds and play essentially two roles. T...

Galina I. Lepesheva, Tatyana Y. Hargrove, Yuliya Kleshchenko, W. David Nes… in Lipids (2008)

Article

Recent Advances in Sterol Research Presented at the 99th AOCS Annual Meeting & Expo in Seattle Washington, May 2008

Robert A. Moreau, W. David Nes, Thomas J. Bach, Edward J. Parish… in Lipids (2008)

Article

Photoaffinity Labeling and Mutational Analysis of 24-C-Sterol Methyltransferase Defines the AdoMet Binding Site

Photolabeling and site-directed mutagenesis were performed on recombinant Saccharomyces cerevisiae 24-C-sterol methyltransferase (SMT) to elucidate the location and role of active site residues involved in AdoMet...

Pruthvi Jayasimha, W. David Nes in Lipids (2008)

Article

Recent Advances in Sterol Research

Edward J. Parish, Robert A. Moreau, Thomas J. Bach, W. David Nes… in Lipids (2007)

Article

Sterol Biosynthesis Inhibitors: Potential for Transition State Analogs and Mechanism-Based Inactivators Targeted at Sterol Methyltransferase

Sterol biosynthesis inhibitors (SBIs), discovered in the late 1960s and subsequently used commercially to treat ergosterol-dependent fungal diseases, represent a unique drug class targeted at an enzyme in a bi...

Zhihong Song, W. David Nes in Lipids (2007)

Article

Recent advances in steroid research

Edward J. Parish, Robert A. Moreau, Isao Ikeda, W. David Nes, John R. Williams in Lipids (2004)

Article

Disruption of ergosterol biosynthesis, growth, and the morphological transition in Candida albicans by sterol methyltransferase inhibitors containing sulfur at C-25 in the sterol side chain

The sterol substrate analog 25-thialanosterol and its corresponding sulfonium salt were evaluated for their ability to serve as antifungal agents and to inhibit sterol methyltransferase (SMT) activity in Candida ...

Ragu Kanagasabai, Wenxu Zhou, Jialin Liu, Thi Thuy Minh Nguyen… in Lipids (2004)

Article

Recent advances in steroid research

Edward J. Parish, W. David Nes, John R. Williams in Lipids (2002)

Article

Evidence for multiple sterol methyl transferase pathways in Pneumocystis carinii

The sterol composition of Pneumocystis carinii, an opportunistic pathogen responsible for life-threatening pneumonia in immunocompromised patients, was determined. Our purpose was to identify pathway-specific enz...

Wenxu Zhou, Thi Thuy Minh Nguyen, Margaret S. Collins, Melanie T. Cushion… in Lipids (2002)

Article

Chemistry, biochemistry, and function of sterols

John D. Weete, Edward J. Parish, W. David Nes in Lipids (2000)

Article

Biochemistry, chemistry, and function of steroids

Edward J. Parish, W. David Nes in Lipids (1997)

Article

Sterol utilization and metabolism by Heliothis zea

Heliothis zea (corn earworm), an insect that fails to synthesize sterols de novo, was reared on an artificial diet treated with 18 different sterol supplements. Larvea did not develop on a sterol...

W. David Nes, Monica Lopez, Wen Zhou, De-an Guo, Patrick F. Dowd… in Lipids (1997)

Chapter

Antifungal Sterol Biosynthesis Inhibitors

Herbal medicines and related drugs used to treat infective organisms were available in ancient cultures and detailed in the first pharmacopoeia reported in 1146 ad, titled “Antidotarium Niclai” (Grun, 1991). Duri...

De-an Guo, Anil T. Mangla, Wen Zhou, Monica Lopez, Zhonghua Jia… in Cholesterol (1997)

Article

Sterol specificity of theSaccharomyces cerevisiae ERG6 gene product expressed inEscherichia coli

TheERG6 gene fromSaccharomyces cerevisiae has been functionally expressed inEscherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl gr...

Mylavarapu Venkatramesh, De-an Guo, James G. Harman, W. David Nes in Lipids (1996)

Article

Regulation of biosynthesis and function of isopentenoids: An introduction

W. David Nes, Edward J. Parish in Lipids (1995)

Article

Developmental regulation of sterol biosynthesis inZea mays

Sixty-one sterols and pentacyclic triterpenes have been isolated and characterized by chromatographic and spectral methods fromZea mays (corn). Several plant parts were examined; seed, pollen, cultured hypocotyl ...

De-an Guo, M. Venkatramesh, W. David Nes in Lipids (1995)

Article

Comparative studies of metabolism of 4-desmethyl, 4-monomethyl and 4,4-dimethyl sterols inManduca sexta

To investigate the metabolism and possible deleterious effects of 4-methyl and 4,4-dimethyl steroids inManduca sexta, the 4,4-dimethyl sterols lanosterol and cycloartenol, the 4-methyl sterol obtusifoliol and the...

James A. Svoboda, Samir A. Ross, W. David Nes in Lipids (1995)

Article

On the structure, biosynthesis, function and phylogeny of isoarborinol and motiol

The solid-state conformations of the C-3 acetates of two isomeric hopanoids—1, isoarborinol (D∶C-friedo-B¹∶A¹-3β,5α,8α,10β,13β,14α,17β,18α,21β) and 2, motion (D∶C-friedo-B¹∶A¹-neogammacer-7(8)-en-3β-ol[3β,5α,9α,1...

W. David Nes, Rosalind Y. Wong, Jane F. Griffin, William L. Duax in Lipids (1991)