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Article
Open AccessOlfactomedin-4 improves cutaneous wound healing by promoting skin cell proliferation and migration through POU5F1/OCT4 and ESR1 signalling cascades
Olfactomedin-4 (OLFM4) is an olfactomedin-domain-containing glycoprotein, which regulates cell adhesion, proliferation, gastrointestinal inflammation, innate immunity and cancer metastasis. In the present stud...
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Article
Open AccessLong-term maintenance of functional primary human hepatocytes in 3D gelatin matrices produced by solution blow spinning
Solution blow spinning (SBS) has recently emerged as a novel method that can produce nano- and microfiber structures suitable for tissue engineering. Gelatin is an excellent precursor for SBS as it is derived ...
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Article
A human preadipocyte cell strain with multipotent differentiation capability as an in vitro model for adipogenesis
Murine 3T3 cell lines constitute a standard model system for in vitro study of mammalian adipogenesis although they do not faithfully reflect the biology of the human adipose cells. Several human adipose cell ...
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Article
Open AccessHyperproliferation is the main driver of metabolomic changes in psoriasis lesional skin
Systematic understanding of the metabolite signature of diseases may lead to a closer understanding of the disease pathogenesis and ultimately to the development of novel therapies and diagnostic tools. Here w...
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Article
Open AccessDiscovery of increased epidermal DNAH10 expression after regeneration of dermis in a randomized with-in person trial — reflections on psoriatic inflammation
Because molecular memories of past inflammatory events can persist in epidermal cells, we evaluated the long-term epidermal protein expression landscapes after dermal regeneration and in psoriatic inflammation...
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Article
Open AccessA label-retaining but unipotent cell population resides in biliary compartment of mammalian liver
Cells with slow proliferation kinetics that retain the nuclear label over long time periods–the label-retaining cells (LRCs)–represent multipotent stem cells in a number of adult tissues. Since the identity of...
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Article
Open AccessThe alterations in the extracellular matrix composition guide the repair of damaged liver tissue
While the cellular mechanisms of liver regeneration have been thoroughly studied, the role of extracellular matrix (ECM) in liver regeneration is still poorly understood. We utilized a proteomics-based approac...
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Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase
During the past decade, the rapid development of new transgenic and knock-in mouse models has propelled epidermal stem-cell research into “fast-forward mode”. It has become possible to identify and visualize d...
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Article
Open AccessLaminin-rich blood vessels display activated growth factor signaling and act as the proliferation centers in Dupuytren’s contracture
Dupuytren’s contracture (DC) is a chronic fibroproliferative disease of the hand, which is characterized by uncontrolled proliferation of atypical myofibroblasts at the cellular level. We hypothesized that spe...
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Article
Fibroblast growth on micro- and nanopatterned surfaces prepared by a novel sol–gel phase separation method
Physical characteristics of the growth substrate including nano- and microstructure play crucial role in determining the behaviour of the cells in a given biological context. To test the effect of varying the ...
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Article
Lgr5 marks cycling, yet long-lived, hair follicle stem cells
Rune Toftgård and colleagues report that Lgr5, a G protein-coupled receptor recently identified as a marker of intestinal stem cells, marks a population of hair follicle stem cells that is actively proliferati...
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Article
p53-dependent transcription can exhibit both on/off and graded response after genotoxic stress
The p53 protein is a central player in cellular response to DNA damage. Induction of p53 by DNA-damaging agents involves elevation of its steady-state level and activation of its potency as a transcription fac...
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Article
p53 protein accumulation in addition to the transactivation activity is required for p53-dependent cell cycle arrest after treatment of cells with camptothecin
In this study we characterize the connections between p53-dependent G1 cell cycle arrest, transcriptional activation of the protein and the increase of its intracellular steady-state concentration. Several cel...
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Article
Oligomerization of p53 is necessary to inhibit its transcriptional transactivation property at high protein concentration
We have previously shown that transactivation by tumor suppressor protein p53 can be inhibited in vivo at elevated protein concentrations. In this study we characterize the structural requirements of this functio...
