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Article
Circulating microRNA Profiles in Acetaminophen Toxicity
Acetaminophen toxicity has been associated with elevation of microRNAs. The present study was to evaluate overall microRNA profiles and previously identified microRNAs to differentiate acetaminophen (APAP) tox...
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Article
Open AccessThe Pseudomonas aeruginosa accessory genome elements influence virulence towards Caenorhabditis elegans
Multicellular animals and bacteria frequently engage in predator-prey and host-pathogen interactions, such as the well-studied relationship between Pseudomonas aeruginosa and the nematode Caenorhabditis elegans. ...
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Article
Open AccessBiodistribution and function of extracellular miRNA-155 in mice
Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. A...
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Article
Open AccessInhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
Antisense reagents can serve as efficient and versatile tools for studying gene function by inhibiting nucleic acids in vivo. Antisense reagents have particular utility for the experimental manipulation of the ac...
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Article
The evolution of our thinking about microRNAs
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Article
mirWIP: microRNA target prediction based on microRNA-containing ribonucleoprotein–enriched transcripts
A new prediction algorithm for microRNA targets, mirWIP, is presented. The algorithm weights target site features based on their enrichment in an experimentally defined immunoprecipitation dataset and identifi...
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Article
Potent effect of target structure on microRNA function
MicroRNAs (miRNAs) are small noncoding RNAs that repress protein synthesis by binding to target messenger RNAs. We investigated the effect of target secondary structure on the efficacy of repression by miRNAs....
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Article
The functions of animal microRNAs
MicroRNAs (miRNAs) are small RNAs that regulate the expression of complementary messenger RNAs. Hundreds of miRNA genes have been found in diverse animals, and many of these are phylogenetically conserved. Wit...
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Article
Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 25 nucleotides) with probable roles in the regulation of gene expression. In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the...
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Protocol
Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning
MicroRNAs (miRNAs) and other small RNAs can be identified by cloning and sequencing cDNAs prepared from the ∼22-nt fraction of total RNA. Methods are described for the construction of cDNA libraries from small...
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Article
Alternative temporal control systems for hypodermal cell differentiation in Caenorhabditis elegans
IN certain multicellular organisms, genetic regulatory systems that specify the timing of cell division, differentiation and morpho-genesis1–3 must accommodate environmental and physiological contingencies that p...
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Chapter
Genetic Basis for Heterochronic Variation
Genes encode the functional and structural components of cells and hence directly or indirectly define the behavior of individual cells and groups of cells in a develo** system. In this sense the genome of a...
