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Open AccessA panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including Omicron
We are amid the historic coronavirus infectious disease 2019 (COVID-19) pandemic. Imbalances in the accessibility of vaccines, medicines, and diagnostics among countries, regions, and populations, and those in...
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Article
Open AccessSARS-CoV-2 infection triggers paracrine senescence and leads to a sustained senescence-associated inflammatory response
Reports of post-acute COVID-19 syndrome, in which the inflammatory response persists even after SARS-CoV-2 has disappeared, are increasing1, but the underlying mechanisms of post-acute COVID-19 syndrome remain un...
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Article
Identification of a distinct lineage of aviadenovirus from crane feces
Viruses are believed to be ubiquitous; however, the diversity of viruses is largely unknown because of the bias of previous research toward pathogenic viruses. Deep sequencing is a promising and unbiased appro...
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A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses
Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 infl...
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Article
Open AccessExperimental infection of Cynomolgus Macaques with highly pathogenic H5N1 influenza virus through the aerosol route
Several animal models are used to study influenza viruses. Intranasal inoculation of animals with a liquid inoculum is one of the main methods used to experimentally infect animals with influenza virus; howeve...
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Selection of antigenically advanced variants of seasonal influenza viruses
Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antige...
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Article
The host protein CLUH participates in the subnuclear transport of influenza virus ribonucleoprotein complexes
The nucleus is highly compartmentalized yet dynamic. Subnuclear functions are regulated by controlling the subnuclear localization of the nuclear proteins. Influenza viral ribonucleoprotein (vRNP) is replicate...
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Open AccessAmino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian a...
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Open AccessA comprehensive map of the influenza A virus replication cycle
Influenza is a common infectious disease caused by influenza viruses. Annual epidemics cause severe illnesses, deaths, and economic loss around the world. To better defend against influenza viral infection, it...
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Characterization of H7N9 influenza A viruses isolated from humans
Here, biological attributes of two early human isolates of the newly emerged H7N9 influenza viruses are characterized: the potential of these viruses to infect and/or transmit within various animal models is d...
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Open AccessIntegrated network analysis reveals a novel role for the cell cycle in 2009 pandemic influenza virus-induced inflammation in macaque lungs
Annually, influenza A viruses circulate the world causing wide-spread sickness, economic loss, and death. One way to better defend against influenza virus-induced disease may be to develop novel host-based the...
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Open AccessExperimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets
Only four mutations in H5N1 HA are required to enable ferret-to-ferret transmission of a reassortant virus containing the H5 HA and the remaining seven gene segments from a human pandemic H1N1 influenza virus.
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In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
Analysis of a series of clinical isolates of the swine-origin H1N1 influenza virus reveals that in mammalian models (mice, ferrets and macaques) the current pandemic virus is associated with more severe diseas...
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Drosophila RNAi screen identifies host genes important for influenza virus replication
Although several alternative antivirals are available for the treatment of influenza virus infection, the risk that viruses resistant to these drugs will emerge means it is important to continue the search for...
