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Article
Open AccessComparison of ex vivo bioluminescence imaging, Alu-qPCR and histology for the quantification of spontaneous lung and bone metastases in subcutaneous xenograft mouse models
Bioluminescence imaging (BLI) is a non-invasive state-of-the-art-method for longitudinal tracking of tumor cells in mice. The technique is commonly used to determine bone metastatic burden in vivo and also sui...
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Living Reference Work Entry In depth
Lungeninfarkt und Infarktpneumonie
Lungeninfarkt und Infarktpneumonie können als Folge einer akuten Lungenembolie (LE) auftreten und gehen häufiger als eine alleinige LE mit pleuritischen Schmerzen und Hämoptysen einher. Bei V. a. einen Lungeni...
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Living Reference Work Entry In depth
Chronisch thromboembolische pulmonale Hypertonie
Die chronisch thromboembolische pulmonale Hypertonie (CTEPH) ist eine mögliche Folgeerkrankung nach akuter Lungenembolie, bei welcher es infolge organisierter Emboliereste und eines pulmonalvaskulären Umbaus z...
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Article
Open AccessTumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
The immunological composition of the tumor microenvironment has a decisive influence on the biological course of cancer and is therefore of profound clinical relevance. In this study, we analyzed the cooperati...
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Article
Open AccessActivation of CD44/PAK1/AKT signaling promotes resistance to FGFR1 inhibition in squamous-cell lung cancer
Lung cancer is the leading cause of cancer-related deaths worldwide. Fibroblast growth factor receptor 1 (FGFR1) gene amplification is one of the most prominent and potentially targetable genetic alterations in s...
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Protocol
Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice
The formation of bone metastases from solid primary tumors comprises several processes following each other in a sequential order in terms of the metastatic cascade. The most widely used preclinical models of ...
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Article
Open AccessCHD1 loss negatively influences metastasis-free survival in R0-resected prostate cancer patients and promotes spontaneous metastasis in vivo
The outcome of prostate cancer (PCa) patients is highly variable and depends on whether or not distant metastases occur. Multiple chromosomal deletions have been linked to early tumor marker PSA recurrence (bi...
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Article
Open AccessIntegrin alpha-V is an important driver in pancreatic adenocarcinoma progression
Mesothelial E- and P-selectins substantially mediate the intraperitoneal spread of Pancreatic ductal adenocarcinoma (PDA) cells in xenograft models. In the absence of selectins in the host, the integrin subuni...
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Article
Open AccessComplications in TLIF spondylodesis–do they influence the outcome for patients? A prospective two-center study
Transforaminal lumbar interbody fusion (TLIF) is a widely accepted surgical procedure for degenerative disk disease. While numerous studies have analyzed complication rates and risk factors this study investi...
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Article
Open AccessThe initial engraftment of tumor cells is critical for the future growth pattern: a mathematical study based on simulations and animal experiments
Xenograft mouse tumor models are used to study mechanisms of tumor growth and metastasis formation and to investigate the efficacy of different therapeutic interventions. After injection the engrafted cells fo...
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Article
Open AccessFully automated dried blood spot sample preparation enables the detection of lower molecular mass peptide and non-peptide do** agents by means of LC-HRMS
The added value of dried blood spot (DBS) samples complementing the information obtained from commonly routine do** control matrices is continuously increasing in sports drug testing. In this project, a robo...
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Article
Probennahme per Laserablation von Krebsgeweben für Proteomics
Distant metastasis is the major cause of cancer-related death in virtually all tumour types. We used spontaneous metastasis xenograft mouse models to study differences between primary tumours and corresponding...
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Article
Open AccessDevelopment and Characterization of a Spontaneously Metastatic Patient-Derived Xenograft Model of Human Prostate Cancer
Here we describe the establishment and characterization of an AR+, PSMA+, ERG+, PTEN−/−, CHD1+/− patient-derived xenograft (PDX) model termed ‘C5’, which has been developed from a 60 years old patient suffering f...
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Article
Clinical relevance of cytoskeleton associated proteins for ovarian cancer
Ovarian cancer has a high mortality rate and up to now no reliable molecular prognostic biomarkers have been established. During malignant progression, the cytoskeleton is strongly altered. Hence we analyzed i...
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Article
Open AccessDifferential Proteome Analysis of Human Neuroblastoma Xenograft Primary Tumors and Matched Spontaneous Distant Metastases
Metastasis formation is the major cause for cancer-related deaths and the underlying mechanisms remain poorly understood. In this study we describe spontaneous metastasis xenograft mouse models of human neurob...
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Article
Open AccessCEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells
We investigated the functional role of CEACAM1 in a spontaneous metastasis xenograft model of human melanoma in scid mice using BRAF wildtype MeWo cells with and without RNAi mediated knockdown of CEACAM1. Tum...
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Article
Open AccessEffects of multilevel posterior ligament dissection after spinal instrumentation on adjacent segment biomechanics as a potential risk factor for proximal junctional kyphosis: a biomechanical study
Spinous processes and posterior ligaments, such as inter- and supraspinous ligaments are often sacrificed either deliberately to harvest osseous material for final spondylodesis e.g. in deformity corrective su...
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Article
Predictors of shoulder level after spinal fusion in adolescent idiopathic scoliosis
For patients with adolescent idiopathic scoliosis, shoulder balance influences their treatment satisfaction and psychological well-being. Several parameters are known to affect postoperative shoulder balance, ...
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Article
The influence of isolated thoracoplasty on the evolution of pulmonary function after treatment of severe thoracic scoliosis
Isolated thoracoplasty (iTP) on the convex side is performed long time after scoliosis surgery has been performed. ITP is thought to cause a further decline in pulmonary function (PF); however, the amount of d...
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Article
Surgical correction of scoliosis in patients with severe cerebral palsy
There is a lack of data in the literature on surgical correction of severe neuromuscular scoliosis in patients with serious extent of cerebral palsy. The purpose of this retrospective cohort study was to analy...
