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Article
Open AccessDesigned allosteric protein logic
The regulation of protein function by external or internal signals is one of the key features of living organisms. The ability to directly control the function of a selected protein would represent a valuable ...
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Article
Open AccessRegulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage
Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Response of protein secretion based on transcriptional control is rather slow, as it requires transcription, tran...
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Article
Open AccessCoiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the desi...
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Article
Design of fast proteolysis-based signaling and logic circuits in mammalian cells
Cellular signal transduction is predominantly based on protein interactions and their post-translational modifications, which enable a fast response to input signals. Owing to difficulties in designing new uni...
