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Article
Open AccessHigh-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform
Telomeres are specialized nucleoprotein structures at the ends of linear chromosomes. The progressive shortening of steady-state telomere length in normal human somatic cells is a promising biomarker for age-a...
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Article
Open AccessStructural insight into the bulge-containing KRAS oncogene promoter G-quadruplex bound to berberine and coptisine
KRAS is one of the most highly mutated oncoproteins, which is overexpressed in various human cancers and implicated in poor survival. The G-quadruplex formed in KRAS oncogene promoter (KRAS-G4) is a transcription...
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Article
Open AccessMesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms
The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, ...
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Article
Open AccessHoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis
The repression of telomerase activity during cellular differentiation promotes replicative aging and functions as a physiological barrier for tumorigenesis in long-lived mammals, including humans. However, the...
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Article
Open AccessHigh-throughput genoty** of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis
Recent advances in the engineering of sequence-specific synthetic nucleases provide enormous opportunities for genetic manipulation of gene expression in order to study their cellular function in vivo. However, c...
