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Protocol
Studying the Role of Astrocytes at Synapses Using Single-Cell Transcriptomics
The central nervous system (CNS) contains a diverse number of neuronal and non-neuronal cell types that physically interact with one another. It is these physical interactions which underlie many essential fun...
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Article
Pharmacological targeting of netrin-1 inhibits EMT in cancer
Epithelial-to-mesenchymal transition (EMT) regulates tumour initiation, progression, metastasis and resistance to anti-cancer therapy1–7. Although great progress has been made in understanding the role of EMT and...
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Article
Methods and applications for single-cell and spatial multi-omics
The joint analysis of the genome, epigenome, transcriptome, proteome and/or metabolome from single cells is transforming our understanding of cell biology in health and disease. In less than a decade, the fiel...
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Article
Dedicated macrophages organize and maintain the enteric nervous system
Correct development and maturation of the enteric nervous system (ENS) is critical for survival1. At birth, the ENS is immature and requires considerable refinement to exert its functions in adulthood2. Here we d...
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Protocol
Single-Cell Genomic and Epigenomic DNA Analyses
This chapter will be dedicated to methods for single-cell genome and epigenome analysis of the human brain. Examining the (epi)genome at a high resolution and sensitivity, allowing for the detection of heterog...
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Article
Open AccessEnhanced chromatin accessibility contributes to X chromosome dosage compensation in mammals
Precise gene dosage of the X chromosomes is critical for normal development and cellular function. In mice, XX female somatic cells show transcriptional X chromosome upregulation of their single active X chrom...
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Article
Open AccessPublisher Correction: LifeTime and improving European healthcare through cell-based interceptive medicine
A Correction to this paper has been published: https://doi.org/10.1038/s41586-021-03287-8.
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Article
Open AccessCommon clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients
Regulatory CD4+ T cells (Treg) prevent tumor clearance by conventional T cells (Tconv) comprising a major obstacle of cancer immune-surveillance. Hitherto, the mechanisms of Treg repertoire formation in human can...
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Article
LifeTime and improving European healthcare through cell-based interceptive medicine
Here we describe the LifeTime Initiative, which aims to track, understand and target human cells during the onset and progression of complex diseases, and to analyse their response to therapy at single-cell re...
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Article
Mechanisms of stretch-mediated skin expansion at single-cell resolution
The ability of the skin to grow in response to stretching has been exploited in reconstructive surgery1. Although the response of epidermal cells to stretching has been studied in vitro2,3, it remains unclear how...
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Article
Heterotypic cell–cell communication regulates glandular stem cell multipotency
Glandular epithelia, including the mammary and prostate glands, are composed of basal cells (BCs) and luminal cells (LCs)1,2. Many glandular epithelia develop from multipotent basal stem cells (BSCs) that are rep...
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Article
Open AccessIdentification of region-specific astrocyte subtypes at single cell resolution
Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood–brain barrier formation, and regulation of ...
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Article
In vitro fertilization does not increase the incidence of de novo copy number alterations in fetal and placental lineages
Although chromosomal instability (CIN) is a common phenomenon in cleavage-stage embryogenesis following in vitro fertilization (IVF)1–3, its rate in naturally conceived human embryos is unknown. CIN leads to mosa...
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Article
Publisher Correction: Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures
In the version of this Technical Report originally published, the competing interests statement was missing. The authors declare no competing interests; this statement has now been added in all online versions...
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Article
Publisher Correction: Early lineage segregation of multipotent embryonic mammary gland progenitors
In the version of this Article originally published, ref. 52 was incorrectly only attributed to its corresponding author, Fre, S., and an older title was used. The correct citation should have been: Lilja, A. ...
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Article
Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures
Embryonic stem cells can be incorporated into the develo** embryo and its germ line, but, when cultured alone, their ability to generate embryonic structures is restricted. They can interact with trophoblast...
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Article
Publisher Correction: A TRP channel trio mediates acute noxious heat sensing
In this Letter, the trace is missing in Fig. 1e. This error has been corrected online.
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Article
Early lineage segregation of multipotent embryonic mammary gland progenitors
The mammary gland is composed of basal cells and luminal cells. It is generally believed that the mammary gland arises from embryonic multipotent progenitors, but it remains unclear when lineage restriction oc...
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Article
Open AccessSingle-cell sequencing reveals the origin and the order of mutation acquisition in T-cell acute lymphoblastic leukemia
Next-generation sequencing has provided a detailed overview of the various genomic lesions implicated in the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL). Typically, 10–20 protein-altering lesio...
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Article
Identification of the tumour transition states occurring during EMT
In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT o...
