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Article
Production of platelet-activating factor by human normodense and hypodense eosinophils
Normodense eosinophils and neutrophils from normal donors produced considerable amounts of plateletactivating factor (PAF) when stimulated with ionophore A23187. PAF produced by eosinophils appeared to be degr...
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Article
Enhancement of substrate recognition ability by combinatorial mutation of β-glucosidase displayed on the yeast cell surface
Recently, in family 3 β-glucosidase (BGL), the catalytically important Asp nucleophile has been identified in the SDW segment of the SDWG sequence by site-directed mutagenesis. However, the details about the r...
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Article
Improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain
To utilize glucoamylase-displaying yeast cells for enzymatic desizing of starched cotton cloth, we constructed yeast strains that codisplayed Rhizopus oryzae glucoamylase and two kinds of Trichoderma reesei cellu...
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Article
Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library
Chitosanase from Paenibacillus fukuinensis D2 is an attractive enzyme, and it exhibits both chitosanase and β-1, 4 glucanase activities. In our previous study, we generated P. fukuinensis chitosanase-displaying y...
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Article
Open AccessImprovement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system
Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and t...
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Article
Overseas migration of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae), from May to mid-July in East Asia
Spodoptera litura (Fabricius) males were monitored with sex-pheromone traps from spring to early summer in 1999, 2002, 2003, 2009, and 2010 in four regions in East Asia: western Japan, South Kore...
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Article
Key-factors and key-stages that determine the leaf longevity of an evergreen broad-leaved tree, Neolitsea sericea (Lauraceae) at different growing sites in southern Japan
Chronological distribution pattern of leaves is an important factor to determine plant productivity in relation to photosynthesis. Therefore, intensive studies of leaf longevity are essential in plant ecology....
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Article
Effects of direct or indirect treatment with insecticides on adult tea leafroller, Caloptilia theivora (Lepidoptera: Gracillariidae), and its progeny
We investigated the effects of the direct and indirect application of insecticides on adults and progenies of the tea leafroller, Caloptilia theivora (Walsingham) (Lepidoptera: Gracillariidae), a serious pest of ...