22 Result(s)
within
Tae-** Yang
Life Sciences
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
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Article
Improved biomass and metabolite production in hairy root culture in various genotypes of Panax ginseng through genetic transformation
The economic important medicinal plant, Panax ginseng, encompasses a diverse array of pharmacologically beneficial ginsenosides governed by complex metabolic pathways. The cultivation of P. ginseng over an extend...
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Article
QTL map** in Vigna radiata × Vigna umbellata population uncovers major genomic regions associated with bruchid resistance
Mungbean (Vigna radiata), genus Vigna, is an economically important legume crop that is valued for its protein-rich dry seeds. However, bruchid infestation causes severe threat to seed storage in terms of deterio...
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Article
Immunogenicity of an S1D epitope from porcine epidemic diarrhea virus and cholera toxin B subunit fusion protein transiently expressed in infiltrated Nicotiana benthamiana leaves
Porcine epidemic diarrhea virus (PEDV) belongs to the Coronaviridae family and causes acute enteritis in pigs. A fragment of the large spike glycoprotein, termed the S1D epitope (aa 636–789), alone and fused with...
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Article
Complete genome sequencing and analysis of Capsicum annuum varieties
Peppers (Capsicum spp.), economically important crops of the nightshade family, are used as vegetables, spices, and medicines. Bacterial wilt (BW), one of the most devastating bacterial plant diseases, causes hea...
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Article
Production of monoclonal antibodies against 53-kDa protein of Porphyromonas gingivalis in transgenic rice cell suspension culture
Periodontitis is a bacterial infection-induced inflammatory disease that affects the tissues surrounding the teeth and is the main cause of tooth loss in adults. Porphyromonas gingivalis, a black-pigmented gram-n...
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Article
Impact of the consecutive days of visible wilting on growth and yield during tassel initiation in maize (Zea Mays L.)
Maize (Zea mays L.) is a major crop with different uses but shows significant susceptibility to drought stress. Recent climate change has caused prolonged drought stress, but in most countries maize production re...
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Yield of maize (Zea mays L.) logistically declined with increasing length of the consecutive visible wilting days during flowering
Under future climate conditions, the frequency and severity of drought are expected to increase. Maize (Zea mays L.) is susceptible to drought, especially at flowering. The objective of this study was to investig...
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Article
High expression of consensus dengue virus envelope glycoprotein domain III using a viral expression system in tobacco
Although plant-based vaccines have many advantages, their use is limited by low expression of antigen genes in transgenic plants, which results in low immune responses and immune tolerance. To overcome this p...
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Article
Production of monoclonal antibody against FimA protein from Porphyromonas gingivalis in rice cell suspension culture
Major fimbriae (FimA) of Porphyromonas gingivalis play an important role in the adherence of the bacterium to oral surfaces, making them a target for the development of a vaccine against periodontal disease. In o...
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Article
Optimal protocol for mass propagation of Aloe vera
We established an advanced protocol for in vitro propagation of Aloe vera via comparison of basal media, sucrose contents, growth hormone combinations, and additional supplementation with various polyamines. The ...
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Article
Amylase and cysteine proteinase gene knockdown in rice cells using RNA interference for enhancing production of recombinant proteins
A rice cell suspension culture with the rice α-amylase 3D promoter expression system which is induced by sucrose starvation was previously reported to generate a good yield of recombinant proteins. However, th...
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Article
Expression of a cholera toxin B subunit and consensus dengue virus envelope protein domain III fusion gene in transgenic rice callus
Dengue (DEN) is one of the most important emerging mosquito-borne viral human diseases. Therefore, an effective dengue vaccine with immune responses against all four dengue virus serotypes is highly needed. A ...
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Article
Expression of a consensus dengue virus envelope protein domain III in transgenic callus of rice
An effective dengue vaccine should elicit immune responses against all four different dengue virus serotypes. This study optimized the codon usage of a gene encoding consensus dengue virus envelope protein dom...
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Article
Expression and assembly of ApxIIA toxin of Actinobacillus pleuropneumoniae fused with the enterotoxigenic E. coli heat-labile toxin B subunit in transgenic tobacco
Actinobacillus pleuropneumoniae is a major etiological agent of swine pleuropneumonia, a highly contagious respiratory infection that causes severe economic losses in the swine production industry. ApxIIA, one of...
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Article
Expression of the Escherichia coli heat-labile enterotoxin B subunit in transgenic watercress (Nasturtium officinale L.)
A gene encoding the B subunit of the enterotoxigenic Escherichia coli heat-labile enterotoxin (LTB) was adapted to the optimized plant coding sequence, and fused to the endoplasmic reticulum retention signal SEKD...
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Article
In silico-selection of Brassica rapa organelle genome-derived BACs using their end sequences and sequence level comparative analysis of the 124 kb mitochondrial genome sequences in the family Brassicaceae
We identified BAC clones which harbor DNAs derived from the B. rapa organelle genomes by in silico map** of 80,292 B. rapa BAC end sequences on the Arabidopsis organelle genomes and subsequent insert size estim...
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Article
Retrotransposons - a major driving force in plant genome evolution and a useful tool for genome analysis
As a major part of most plant genomes, retrotransposons are distributed throughout the plant genome ubiquitously with high copy number and extensive heterogeneity. Various retrotransposon families with distinc...
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Article
Expression of a cholera toxin B subunit in transgenic lettuce (Lactuca sativa L.) using Agrobacterium-mediated transformation system
To increase expression level of cholera toxin B subunit (CTB) in lettuce plants, synthetic CTB (sCTB) gene based on the optimized codon usage was fused with an endoplasmic reticulum retention signal, KDEL. The...
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Article
Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants
Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. A neutralizing epitope of porcine epidemic diarrhea virus...
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Article
Expression of the B subunit of E. coli heat-labile enterotoxin in tobacco using a herbicide resistance gene as a selection marker
The B subunit of Escherichia coli heat-labile enterotoxin (LTB) has been transformed to plants for use as an edible vaccine. We have developed a simple and reliable Agrobacterium-mediated transformation method to...
