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Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants

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Abstract

Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. A neutralizing epitope of porcine epidemic diarrhea virus (PEDV) gene containing SEKDEL was expressed in potato using Agrobacterium-mediated transformation system. Putative transgenic plants were regenerated, and genomic PCR confirmed the presence of PEDV epitope gene in the potato plants. Based on the ELISA results, epitope of PEDV protein made up approximately 0.1% of the total soluble tuber protein.

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Abbreviations

ELISA:

enzyme linked immunosorbent assay

PEDV:

porcine epidemic diarrhea virus

TSP:

total soluble protein

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Authors and Affiliations

  1. Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University, South Korea

    Young-Sook Kim

  2. Institute of Basic Science, Chonbuk National University, South Korea

    Tae-** Kang

  3. Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University, Jeonju, Chonbuk, 561–756, South Korea

    Yong-Suk Jang & Moon-Sik Yang

Authors
  1. Young-Sook Kim
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  2. Tae-** Kang
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  3. Yong-Suk Jang
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  4. Moon-Sik Yang
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Correspondence to Moon-Sik Yang.

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Kim, YS., Kang, TJ., Jang, YS. et al. Expression of neutralizing epitope of porcine epidemic diarrhea virus in potato plants. Plant Cell Tiss Organ Cult 82, 125–130 (2005). https://doi.org/10.1007/s11240-004-7203-7

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  • DOI: https://doi.org/10.1007/s11240-004-7203-7

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