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Article
Open AccessThe assembly of the Mitochondrial Complex I Assembly complex uncovers a redox pathway coordination
The Mitochondrial Complex I Assembly (MCIA) complex is essential for the biogenesis of respiratory Complex I (CI), the first enzyme in the respiratory chain, which has been linked to Alzheimer’s disease (AD) p...
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Article
Open AccessStructural landscape of the respiratory syncytial virus nucleocapsids
Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffol...
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Article
Open AccessEGOC inhibits TOROID polymerization by structurally activating TORC1
Target of rapamycin complex 1 (TORC1) is a protein kinase controlling cell homeostasis and growth in response to nutrients and stresses. In Saccharomyces cerevisiae, glucose depletion triggers a redistribution of...
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Article
Structural basis of CHMP2A–CHMP3 ESCRT-III polymer assembly and membrane cleavage
The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, the struc...
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Article
Open AccessStructural basis of tankyrase activation by polymerization
The poly-ADP-ribosyltransferase tankyrase (TNKS, TNKS2) controls a wide range of disease-relevant cellular processes, including WNT–β-catenin signalling, telomere length maintenance, Hippo signalling, DNA dama...
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Article
Open AccessStructural and biochemical characterisation of the Providencia stuartii arginine decarboxylase shows distinct polymerisation and regulation
Bacterial homologous lysine and arginine decarboxylases play major roles in the acid stress response, physiology, antibiotic resistance and virulence. The Escherichia coli enzymes are considered as their archetyp...
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Article
Open AccessComplete structure of the chemosensory array core signalling unit in an E. coli minicell strain
Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise st...
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Article
Atomic structures of an entire contractile injection system in both the extended and contracted states
Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, bacterial ...
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Article
Open AccessMolecular architecture and regulation of BCL10-MALT1 filaments
The CARD11-BCL10-MALT1 (CBM) complex triggers the adaptive immune response in lymphocytes and lymphoma cells. CARD11/CARMA1 acts as a molecular seed inducing BCL10 filaments, but the integration of MALT1 and t...
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Article
Open AccessRIP2 filament formation is required for NOD2 dependent NF-κB signalling
Activation of the innate immune pattern recognition receptor NOD2 by the bacterial muramyl-dipeptide peptidoglycan fragment triggers recruitment of the downstream adaptor kinase RIP2, eventually leading to NF-...
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Article
TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity
In budding yeast, glucose withdrawal, via the Rag GTPases, leads to TORC1 inhibition through its re-organization into a giant, vacuole-associated helix named a TOROID (TORC1 organized in inhibited domain).
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Article
Open AccessIn vitro assembly of the Rous Sarcoma Virus capsid protein into hexamer tubes at physiological temperature
During a proteolytically-driven maturation process, the orthoretroviral capsid protein (CA) assembles to form the convex shell that surrounds the viral genome. In some orthoretroviruses, including Rous Sarcoma...
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Article
Self-organization of the vesicular stomatitis virus nucleocapsid into a bullet shape
The typical bullet shape of Rhabdoviruses is thought to rely on the matrix protein for stabilizing the nucleocapsid coil. Here we scrutinize the morphology of purified and recombinant nucleocapsids of vesicula...
