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  1. Article

    Open Access

    Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

    Sorghum (Sorghum bicolor L.) is one of the world’s most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has be...

    Srinivas Belide, Thomas Vanhercke, James Robertson Petrie in Plant Methods (2017)

  2. No Access

    Article

    Rapid expression and validation of seed-specific constructs in transgenic LEC2 induced somatic embryos of Brassica napus

    Seed-specific modification of crop plants is a common goal in plant metabolic engineering. Ability for early assessment and validation of seed-specific constructs for metabolic engineering in crop plants is de...

    Srinivas Belide, Xue-Rong Zhou, Yoko Kennedy in Plant Cell, Tissue and Organ Culture (PCTO… (2013)

  3. No Access

    Article

    Transgenic production of arachidonic acid in oilseeds

    We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both ...

    James R. Petrie, Pushkar Shrestha, Srinivas Belide, Maged P. Mansour in Transgenic Research (2012)

  4. Article

    Open Access

    Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting

    Safflower (Carthamus tinctorius L.) is a difficult crop to genetically transform being susceptible to hyperhydration and poor in vitro root formation. In addition to traditional uses safflower has recently emerge...

    Srinivas Belide, Luch Hac, Surinder P Singh, Allan G Green, Craig C Wood in Plant Methods (2011)