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Article
Rapid expression and validation of seed-specific constructs in transgenic LEC2 induced somatic embryos of Brassica napus
Seed-specific modification of crop plants is a common goal in plant metabolic engineering. Ability for early assessment and validation of seed-specific constructs for metabolic engineering in crop plants is de...
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Article
Transgenic production of arachidonic acid in oilseeds
We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both ...
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Article
Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production
The production of long-chain polyunsaturated fatty acids from precursor molecules linoleic acid (LA; 18:2ω6) and α-linolenic acid (ALA; 18:3ω3) is catalysed by sequential desaturase and elongase reactions. We ...
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Article
Open AccessRapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production
Metabolic engineering of seed biosynthetic pathways to diversify and improve crop product quality is a highly active research area. The validation of genes driven by seed-specific promoters is time-consuming s...
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Article
Isolation and Characterisation of a Δ5-fatty Acid Elongase from the Marine Microalga Pavlova salina
The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic a...
