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Article
Open AccessParadoxical effects of elastase inhibitor guamerin on the tissue repair of two different wound models: sealed cutaneous and exposed tongue wounds
Innate elastase inhibitors are known to be putatively involved in the regulation of tissue inflammation by inhibiting polymorphonuclear leukocyte (PMN) derived proteinases. The aim of this study was to evaluat...
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Article
Open AccessDifferential inhibition of endothelial cell proliferation and migration by urokinase subdomains: amino-terminal fragment and kringle domain
The serine protease urokinase-type plasminogen activator (uPA) is implicated in pericellular proteolysis in a variety of physiological and pathological processes including angiogenesis and tumor metastasis. Th...
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Article
Open AccessProtein kinase CK2 phosphorylates and interacts with deoxyhypusine synthase in HeLa cells
Deoxyhypusine is a modified lysine and formed posttranslationally to be the eukaryotic initiation factor eIF5A by deoxyhypusine synthase, employing spermidine as butylamine donor. Subsequent hydroxylation of t...
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Article
Open AccessDeoxyhypusine synthase is phosphorylated by protein kinase C in vivo as well as in vitro
Deoxyhypusine synthase catalyzes the first step in the posttranslational synthesis of an unusual amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF-5A) precursor protein. We earlier ...
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Article
Open AccessEffects of Val34Leu and Val35Leu polymorphism on the enzyme activity of the coagulation factor XIII-A
Change in fibrin stabilizing activity of factor XIII A subunit (FXIII-A) caused by a specific mutation, Val34Leu, is recently implicated to incidences of pathophysiology of thrombosis. In an effort to understa...
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Article
Attachment characteristics of normal human cells and virus-infected cells on microcarriers
The attachment kinetics of normal and virus-infected LuMA cells were studied to improve the production of live attenuated varicella viruses in human embryonic lung (LuMA) cells. Normal LuMA cells and LuMA cell...
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Article
Cloning and Characterization of a 22 kDa Outer-Membrane Protein (Omp22) from Helicobacter pylori
Helicobacter pylori is a causative agent of gastritis and peptic ulceration in humans. As the first step towards development of a vaccine against H. pylori infection, we have attempted to identify protective anti...
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Article
Open AccessThe mucosal adjuvanticity of two nontoxic mutants of Escherichia coli heat-labile enterotoxin varies with immunization routes
Escherichia coli heat-labile enterotoxin (LT), which causes a characteristic diarrhea in humans and animals, is a strong mucosal immunogen and has powerful mucosal adjuvant activity towards coadministered unre...
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Article
Open AccessPurification and characterization of recombinant murine endostatin in E. coli
Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor. We rep...
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Article
Open AccessCharacterization of yeast deoxyhypusine synthase: PKC-dependent phosphorylation in vitro and functional domain identification
The biosynthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)-lysine] occurs in the eIF-5A precursor protein through two step posttranslational modification involving deoxyhypusine synthase which catalyzes transfe...
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Article
Open AccessThe inhibitory effects of recombinant plasminogen kringle 1-3 on the neovascularization of rabbit cornea induced by angiogenin, bFGF, and VEGF
Angiostatin is a potent angiogenesis inhibitor that is composed of the first four kringles of plasminogen fragment. Angiostatin with one less kringle molecule (kringle 1 to 3) was recently demonstrated to be a...
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Article
Open AccessDevelopment of two novel nontoxic mutants of Escherichia coli heat-labile enterotoxin
Escherichia coli heat-labile enterotoxin (LT) is composed of catalytic A and non-catalytic homo-pentameric B subunits and causes diarrheal disease in human and animals. In order to produce a nontoxic LT for va...
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Article
Effect of dehydration and rehydration of the pH-sensitive liposomes containing chimeric gag-V3 virus like particle on their long-term stability
One of the practical limitations with the use of liposomes for delivery of the pharmaceutical substances such as antigens is that liposomes are relatively unstable in storage. In order to extend the stability ...
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Macrophage Transglutaminases: Characterization of Molecular Species and Measurement of Enzymatic Modification by Cigarette Smoke Components
Transglutaminases (E.C. 2.3.2.13) are a family of calcium-dependent thiol enzymes which catalyze the covalent cross-linking of peptide-bound glutamine residues to a variety of primary amines, including the eps...
