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Chapter
Immunological Disorders: Regulation of Ca2+ Signaling in T Lymphocytes
Engagement of T cell receptors (TCRs) with cognate antigens triggers cascades of signaling pathways in helper T cells. TCR signaling is essential for the effector function of helper T cells including prolifera...
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Article
Orai1-NFAT signalling pathway triggered by T cell receptor stimulation
T cell receptor (TCR) stimulation plays a crucial role in development, homeostasis, proliferation, cell death, cytokine production, and differentiation of T cells. Thus, in depth understanding of TCR signallin...
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Protocol
Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes
Measurement of intracellular Ca2+ concentration ([Ca2+]i) is useful to study the upstream and downstream events of Ca2+ signaling. Ca2+-binding proteins including EF-hand-containing proteins are important downstr...
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Article
A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells
The calcium-binding protein CRACR2A positively regulates the activity of CRAC channels. CRACR2A is a ternary complex which binds to, and stabilizes, the interaction between STIM1 and Orai1, and disassociates a...