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  1. Article

    Open Access

    High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

    pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the appl...

    **ngYa Zhao, JianYong Xu, Ming Tan, Jie Zhen, WenJu Shu… in Microbial Cell Factories (2020)

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  2. No Access

    Article

    High-level extracellular production of an alkaline pectate lyase in E. coli BL21 (DE3) and its application in bioscouring of cotton fabric

    A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in E. coli was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by E...

    Jie Zhen, Ming Tan, ** Fu, Wenju Shu, **ngya Zhao, Shibin Yang… in 3 Biotech (2020)