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  1. Article

    Open Access

    Flow cytometric analysis and sorting of plant chromosomes

    Flow cytogenetics is a high-throughput technique that classifies large populations of mitotic chromosomes according to their fluorescence and light scattering as they move in a single file rapidly in a narrow ...

    Jaroslav Doležel, Petr Urbiš, Mahmoud Said, Sergio Lucretti, István Molnár in The Nucleus (2023)

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  2. No Access

    Chapter

    Cytological and Molecular Cytogenetic Insights into the Cynara cardunculus Genome

    Before the recent advances in molecular biology and sequencing techniques enabled a deep knowledge of the complete nucleotide sequence of many plant genomes, cytological and karyological studies have provided ...

    Debora Giorgi, Gianmarco Pandozy, Anna Farina… in The Globe Artichoke Genome (2019)

  3. No Access

    Chapter

    FISHIS: A New Way in Chromosome Flow Sorting Makes Complex Genomes More Accessible

    Eukaryotic chromosomes can be studied either fixed on slides by use of cytogenetic techniques or through flow-cytometry. The latter enables not only the chromosome characterization but allows for the sorting a...

    Sergio Lucretti, Debora Giorgi, Anna Farina… in Genomics of Plant Genetic Resources (2014)

  4. No Access

    Article

    Assessment of the origin of new citrus tetraploid hybrids (2n = 4x) by means of SSR markers and PCR based dosage effects

    We report the accurate determination of the allelic configurations of a total of eight new citrus tetraploid hybrids by means of SSR analysis, coupled with capillary electrophoresis, and PCR based dosage effec...

    Sergio Pietro Ferrante, Sergio Lucretti, Silvia Reale, Alessandro De Patrizio… in Euphytica (2010)

  5. No Access

    Chapter

    Large Particle Sorting

    The design of jet-in-air flow sortes has centered around the optimal analysis of mammalian cells, particularly those of the lymphoid system, for sensible reasons of commerce. Lymphoid cells have small diameter...

    David W. Galbraith, Sergio Lucretti in Flow Cytometry and Cell Sorting (2000)

  6. No Access

    Article

    Bivariate flow cytometry DNA/BrdUrd analysis of plant cell cycle

    We describe a protocol for flow cytometry analysis of cell cycle in plants using indirect immunolabelling staining and Vicia faba, Pisum sativum and Zea mays root tip cells as model systems. The protocol is ba...

    Sergio Lucretti, Luca Nardi, Paola Trionfetti Nisini… in Methods in Cell Science (1999)

  7. No Access

    Article

    Localization of seed protein genes on flow-sorted field bean chromosomes

    Chromosomes from reconstructed field bean (Vicia faba L.) karyotypes were flow-sorted and the DNA was used for the physical localization of seed storage and nonstorage (USP) protein genes using PCR with sequence ...

    Jiří Macas, Jaroslav Doležel, Sergio Lucretti, Uta Pich… in Chromosome Research (1993)