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Article
Open AccessCrystal structure of l-2-keto-3-deoxyfuconate 4-dehydrogenase reveals a unique binding mode as a α-furanosyl hemiketal of substrates
l-2-Keto-3-deoxyfuconate 4-dehydrogenase (l-KDFDH) catalyzes the NAD+-dependent oxidization of l-2-keto-3-deoxyfuconate (l-KDF) to l-2,4-diketo-3-deoxyfuconate (l-2,4-DKDF) in the non-phosphorylating l-fucose pat...
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Article
Open AccessInfluence of liquid properties of colloidal crystals to thermal stability of colloidal crystals on different substrate surfaces through heterogeneous nucleation
The effects of liquid properties, such as pH and conductivity, on the thermal stability of electrostatically interacting colloidal silica crystals generated from heterogeneous nuclei were investigated mainly b...
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Article
An experimental study of surface wave effects on two interacting tidal turbines
The development of tidal turbine farms desires an understanding of the effects of free-surface waves and turbine interactions. The objective of this paper is to investigate surface wave effects on the performa...
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Article
Open AccessMolecular evolutionary insight of structural zinc atom in yeast xylitol dehydrogenases and its application in bioethanol production by lignocellulosic biomass
Xylitol dehydrogenase (XDH) catalyzes the NAD+-dependent oxidization of xylitol into d-xylulose, and belongs to a zinc-dependent medium-chain dehydrogenase/reductase family. This protein family consists of enzyme...
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Article
Open AccessHigh Friction Mechanism of ZDDP Tribofilm Based on in situ AFM Observation of Nano-Friction and Adhesion Properties
Zinc dialkyl dithiophosphate (ZDDP) is a typical anti-wear additive that forms tribofilm on sliding surfaces and prevents adhesion wear but increases the friction force. In this study, the nano-friction and ad...
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Article
Lattice Boltzmann simulations for multiple tidal turbines using actuator line model
In numerical simulations of tidal current farms, large-scale computational fluid dynamic (CFD) simulations with a high-resolution grid are required to calculate the interactions between tidal turbines. In this...
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Article
Open AccessProcesses of molecular adsorption and ordering enhanced by mechanical stimuli under high contact pressure
Adsorbed molecular films, referred to as boundary films in tribology, are widely used in various industrial products as a keyway for surface functionalisation, such as lubricity, wettability, and adhesion. Bec...
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Article
Durability Improvement of Concentrated Polymer Brushes by Multiscale Texturing
Concentrated polymer brushes (CPBs) are promising soft-material coatings for improving tribological properties under severe sliding conditions, even in the macroscopic scale. Therefore, they are expected to be...
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Article
Open AccessCrystal structures of aconitase X enzymes from bacteria and archaea provide insights into the molecular evolution of the aconitase superfamily
Aconitase superfamily members catalyze the homologous isomerization of specific substrates by sequential dehydration and hydration and contain a [4Fe-4S] cluster. However, monomeric and heterodimeric types of ...
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Article
Numerical simulation of dam break flow impact on vertical cylinder by cumulant lattice Boltzmann method
The purpose of this study is to apply the cumulant lattice Boltzmann method (LBM) to simulate high Reynolds number free surface flows. The single-phase cumulant LBM is extended to solve the free surface flows ...
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Article
Open AccessNovel non-phosphorylative pathway of pentose metabolism from bacteria
Pentoses, including D-xylose, L-arabinose, and D-arabinose, are generally phosphorylated to D-xylulose 5-phosphate in bacteria and fungi. However, in non-phosphorylative pathways analogous to the Entner-Dodoro...
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Article
Lubricating property of cyano-based ionic liquids against hard materials
Ionic liquids are expected to be used as a new lubricants and lubricant additives because of their unique properties. However, cyano-based ionic liquids have exhibited poor lubricating property with steel/stee...
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Article
Effects of Alkyl Chain Length of Sulfate and Phosphate Anion-Based Ionic Liquids on Tribochemical Reactions
Ionic liquids are expected to become increasingly popular lubricants as they feature a number of attractive properties. This investigation focused on sulfate and phosphate anion-based ionic liquids and the imp...
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Article
Open AccessFunctional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase
In the aconitase superfamily, which includes the archetypical aconitase, homoaconitase, and isopropylmalate isomerase, only aconitase X is not functionally annotated. The corresponding gene (LhpI) was often locat...
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Article
Breeding of a xylose-fermenting hybrid strain by mating genetically engineered haploid strains derived from industrial Saccharomyces cerevisiae
The industrial Saccharomyces cerevisiae IR-2 is a promising host strain to genetically engineer xylose-utilizing yeasts for ethanol fermentation from lignocellulosic hydrolysates. Two IR-2-based haploid strains w...
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Article
Tribochemical Reactions of Ionic Liquids Under Vacuum Conditions
Ionic liquids are expected to function as novel lubricants owing to their attractive characteristics such as high thermal stability and low vapor pressure. In order for ionic liquids to be used as lubricants,...
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Article
RNAi Knockdown of Potent Sugar Sensor in Cellulase-Producing Fungus Acremonium cellulolyticus
A potent sugar sensor gene (g9105) was screened from the genomic data of the cellulase-producing fungus Acremonium cellulolyticus. The transcriptional level of g9105 in the SA49 transformant, which carried the kn...
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Article
Molecular Behavior of Room-temperature Ionic Liquids under Lubricating Condition
The molecular behavior of room-temperature ionic liquids (RTILs) under lubricating condition was analyzed using fourier transform infrared spectroscopy equipped with a pin-on-disk tribometer to simultaneously ...
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Chapter and Conference Paper
Construction of a Novel Strictly NADPH-Dependent Pichia stipitis Xylose Reductase by Site-Directed Mutagenesis for Effective Bioethanol Production
Xylose reductase (XR) is one of the key enzymes for bio-ethanol production from lignocellulosic biomass. Intercellular redox imbalance, caused by different coenzyme specificity of XR and Xylitol dehydrogenase ...
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Article
Expression of protein engineered NADP+-dependent xylitol dehydrogenase increases ethanol production from xylose in recombinant Saccharomyces cerevisiae
A recombinant Saccharomyces cerevisiae strain transformed with xylose reductase (XR) and xylitol dehydrogenase (XDH) genes from Pichia stipitis has the ability to convert xylose to ethanol together with the unfav...
