Enzyme Immunoassays
From Concept to Product Development
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S.S. Deshpande
Food Science
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Book
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Chapter
Antibodies: Biochemistry, Structure, and Function
An understanding of the basic principles of the biology of the vertebrate immune system and its regulation is a prerequisite for the successful experimental production of antibodies. Such knowledge helps in av...
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Chapter
Conjugation Techniques
Preparation of well-defined conjugates during various stages, from hapten immunogen preparation for antibody production to that of tracer or labeling reagents such as enzyme-antigen or enzyme-antibody conjugat...
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Enzymes and Signal Amplification Systems
Since the introduction of radioimmunoassays in the late 1950s and the widespread reliance on radioisotopic labels in various fields of clinical and medical science and practice during the 1960s and 1970s, rapi...
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Immunoassay Classification and Commercial Technologies
Immunoassays encompass techniques for the detection and quantification of antigens or antibodies, and are one of the most powerful of all immunochemical techniques. Although the term “immunoassay” generally re...
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Data Analysis
Data analysis is an important aspect of using solid-phase immunoassays. The basic principles of obtaining dose-response curves using immunoassay techniques were described earlier in this book. In quantitative ...
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Introduction
Qualitative and quantitative analytical techniques are of tremendous importance in all fields and disciplines of life sciences for the detection, identification, and measurement of concentration of a wide vari...
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Antigen-Antibody Reactions
Enzyme immunoassays are based on antigen-antibody reactions involving enzyme-labeled antigen (or antibody) with antibody (or antigen). An understanding of the fundamentals of antigen-antibody reactions is, the...
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Chapter
Antibody Production
The production and large scale availability of antibody of desired affinity and specificity and/or cross-reactivity are fundamental to the process of develo** sensitive, precise, and specific immunoassay pro...
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Separation and Solid-Phase Systems
To quantitate the amount of analyte present in an enzyme immunoassay (EIA), the extent of reaction of the enzyme-labeled ligand (or antibody) with antibody (or the ligand) must be determined. Since the reactio...
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Assay Development, Evaluation, and Validation
Modern immunoassay systems exist in several different formats with diverse ancillary technologies. An ideal immunoassay for a given need depends upon the kind of information desired and the physical environmen...
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Chapter
Reagent Formulations and Shelf Life Evaluation
The control of assay quality and the degree of quality control required begin with the source and the accurate preparation of reagents and control sera used in the assay procedure. The quality of reagents used...
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Documentation, Registration, and Diagnostic Start-Ups
Commercial production of immunodiagnostic kits requires that the various components be manufactured consistently over a long period of time. The process involves availability of a consistent source of antibody...
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Chapter
Introduction
Never in the history of humankind did we face such monumental challenges and daunting prospects as we do approaching the dawn of the twenty-first century. The present world population of about 5 billion and it...
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Chapter
Legumes
Legumes (including soybeans and peanuts) are ranked fifth in terms of annual world grain production, at around 170 million metric tons, after wheat, rice, corn, and barley (Table 2-1; cf. Table 0-1). With the ...
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Chapter
Cereals
Cereal grains are the major food of humankind. In many of the less developed countries of the world, cereals provide two-thirds or more of the dietary calories (Deshpande and Damodaran 1990). Rice in Asia, cor...
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Article
Water uptake during cooking of dry beans (Phaseolus vulgaris L.)
Water uptake during cooking of ten dry bean (Phaseolus vulgaris L.) varieties was investigated. Water uptake during early stages of cooking was characteristic of the variety. Although the optimal cooking times va...
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Article
Interrelationships between certain physical and chemical properties of dry bean (Phaseolus vulgaris L.)
The interrelationships among certain physical and chemical properties of ten cultivars of dry beans (Phaseolus vulgaris L.) were investigated. The length/breadth ratio was similar (1.51–1.65) except for kidney be...
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Article
X-ray fluorescence method for the determination of rare earths, uranium and thorium in allanites
Das beschriebene Verfahren eignet sich zur Bestimmung von La, Ce, Pr, Nd, Sm Gd, Th und U in Allaniten. Die Bestimmungsgrenzen für die einzelnen Elemente betragen: La 0,5–10%; Ce 2–20%; Pr 0,1–2%; Nd 0,5–10%; ...
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Article
X-ray fluorescence analysis of high-purity dysprosium oxide for Y, Eu, Gd, Tb, Ho and Er
Die Proben werden in Oxalat übergeführt und im Verhältnis 2∶1 mit Borsäure vermischt. Diese Mischung wird als zweite Schicht auf eine Borsäuretablette gepreßt. Die Auswahl der geeigneten Analysenlinien und die...
