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  1. No Access

    Article

    Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture

    Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the fine structural features of cellular compartments. In the present study, this approach has been applied in order t...

    C. Bouchet-Marquis, J. Dubochet, S. Fakan in Histochemistry and Cell Biology (2006)

  2. No Access

    Article

    Ultrastructural analysis of nucleolar transcription in cells microinjected with 5-bromo-UTP

    In situ sites of nucleolar transcription in cells microinjected with 5-bromo-UTP (BrUTP) were visualized at an ultrastructural level. After injection the cells were maintained for 4–90 min at 37°C, fixed, and ...

    D. Cmarko, P. J. Verschure, L. I. Rothblum… in Histochemistry and Cell Biology (2000)

  3. No Access

    Article

    Electron spectroscopic imaging analyses of the distribution of phosphorus in Balbiani ring granules and in the surrounding nucleoplasm

    The in situ distribution of phosphorus was studied in unstained ultrathin sections of salivary glands ofChironomus tentans andCh. thummi larvae using elemental map** by means of an energy-filtering transmission...

    G. H. Vázquez-Nin, S. Abolhassani-Dadras, O. M. Echeverría… in Chromosoma (1997)

  4. No Access

    Article

    Fine structural cytochemical and immunocytochemical analysis of nucleic acids and ribonucleoprotein distribution in nuclei of pig oocytes and early preimplantation embryos

    The fine structure of pig oocytes at the germinal vesicle (GV) stage and early preimplantation embryos (one to four blastomeres) isolated at slaughter was investigated by cytochemical and immunocytochemical me...

    V. Kopecny, M. Biggiogera, J. Laurincik, J. Pivko, P. Grafenau, T. E. Martin… in Chromosoma (1996)

  5. No Access

    Article

    Retention of glycogen in cryosubstituted mouse liver

    A periodic acid-Schiff (PAS)-type reaction in which osmium-ammine was used as the reagent was carried out on ultrathin sections of mouse liver in order to study the extent to which glycogen is preserved. Compa...

    M. L. von Schack, S. Fakan in Histochemistry (1994)

  6. No Access

    Article

    A new type of ribonucleoprotein constituent of the polytene nucleus of the salivary glands ofChironomus thummi andCh. tentans

    Using electron spectroscopic imaging, a new type of small granular structural constituent has been observed in the extrachromosomal zone of the polytene nucleus of the salivary gland cells ofChironomus thummi and

    G. H. Vázquez-Nin, O. M. Echeverría, V. Boutinard Rouelle-Rossier, S. Fakan in Chromosoma (1993)

  7. No Access

    Article

    Immunoelectron microscope localization of Mr 90000 heat shock protein and Mr 70000 heat shock cognate protein in the salivary glands of Chironomus thummi

    The Mr 90000 heat shock protein (hsp 90) and one of the Mr 70000 heat shock cognate proteins (hsc 70) were localized by immunoelectron microscopy in salavary gland cells of normal and heat-shocked larvae of Chiro...

    G. H. Vázquez-Nin, O. M. Echeverría, M. E. Carbajal, R. M. Tanguay… in Chromosoma (1992)

  8. No Access

    Article

    A cytochemical and immunocytochemical study of DNA distribution in spermatid nuclei of mouse, rabbit, and bull

    DNA distribution in mouse, rabbit and bull spermatids was analyzed by electron microscopy, after using a Feulgen-like HCl-osmium ammine procedure, and after immunocytochemistry with anti-DNA antibodies. In add...

    J. L. Courtens, M. Biggiogera, S. Fakan in Cell and Tissue Research (1991)

  9. No Access

    Article

    Distribution of nucleolar proteins B23 and nucleolin during mouse spermatogenesis

    The intracellular distribution of nucleolar phosphoproteins B23 and nucleolin was studied during mouse spermatogenesis, a process that is characterized by a progressive reduction of nucleolar activity. Biochem...

    M. Biggiogera, S. H. Kaufmann, J. H. Shaper, N. Gas, F. Amalric, S. Fakan in Chromosoma (1991)

  10. No Access

    Article

    Immunoelectron microscope localization of snRNPs in the polytene nucleus of salivary glands ofChironomus thummi

    The ultrastructural distribution of small nuclear ribonucleoproteins (U1-snRNP and Sm antigen) in the nucleus ofChironomus salivary gland cells was investigated by means of specific antibodies and immunocytochemi...

    G. H. Vazquez-Nin, O. M. Echeverria, S. Fakan, G. Leser, T. E. Martin in Chromosoma (1990)

  11. No Access

    Article

    Visualization of transcription in early mouse embryos

    Transcriptional activity during early mammalian embryogenesis was examined in 2 cell, 4–8 cell and late morula/early blastocyst mouse embryos. Zona pellucida-free embryos were obtained after pronase digestion ...

    M. E. Hughes, K. Bürki, S. Fakan in Chromosoma (1979)