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Article
Open AccessAuthor Correction: Meta-reinforcement learning via orbitofrontal cortex
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Article
Open AccessSynaptically-targeted long non-coding RNA SLAMR promotes structural plasticity by increasing translation and CaMKII activity
Long noncoding RNAs (lncRNAs) play crucial roles in maintaining cell homeostasis and function. However, it remains largely unknown whether and how neuronal activity impacts the transcriptional regulation of ln...
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Article
Open AccessMeta-reinforcement learning via orbitofrontal cortex
The meta-reinforcement learning (meta-RL) framework, which involves RL over multiple timescales, has been successful in training deep RL models that generalize to new environments. It has been hypothesized tha...
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Article
CaMKII: a central molecular organizer of synaptic plasticity, learning and memory
Calcium–calmodulin (CaM)-dependent protein kinase II (CaMKII) is the most abundant protein in excitatory synapses and is central to synaptic plasticity, learning and memory. It is activated by intracellular in...
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Protocol
FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs
With the development of fluorescent proteins (FPs) and advanced optical microscopy techniques, Förster or fluorescence resonance energy transfer (FRET) has become a powerful tool for real-time noninvasive visu...
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Article
Open AccessRapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research
The Cre/LoxP-based conditional knockout technology is a powerful tool for gene function analysis that allows region- and time-specific gene manipulation. However, inserting a pair of LoxP cassettes to generate...
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Chapter and Conference Paper
Efficient Data Presentation Method for Building User Preference Model Using Interactive Evolutionary Computation
this paper, we propose data presentation methods for building user preference models efficiently in recommendation systems by interactive genetic algorithm. The user preference model of the recommender ...
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Article
Open AccessRac1 is a downstream effector of PKCα in structural synaptic plasticity
Structural and functional plasticity of dendritic spines is the basis of animal learning. The rapid remodeling of actin cytoskeleton is associated with spine enlargement and shrinkage, which are essential for ...
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Protocol
Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography
Live imaging of dendritic spines using advanced light microscopy (LM) provides insight into how the brain processes information to learn and form memories. As a complementary approach, electron microscopy (EM)...
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Article
Open AccessMechanisms of Ca2+/calmodulin-dependent kinase II activation in single dendritic spines
CaMKIIα plays an essential role in decoding Ca2+ signaling in spines by acting as a leaky Ca2+ integrator with the time constant of several seconds. However, the mechanism by which CaMKIIα integrates Ca2+ signals...
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Protocol
Analysis of TrkB Receptor Activity Using FRET Sensors
Here, we describe the use of 2-photon fluorescence lifetime imaging (2pFLIM) of a Förster resonance energy transfer (FRET) sensor to study the spatial and temporal activity pattern of the BDNF receptor TrkB. C...
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Protocol
Imaging Neuronal Signal Transduction Using Multiphoton FRET-FLIM
Synaptic plasticity, the ability of neurons to modulate the strength of specific inputs, is critical for neural circuits to adapt to experience throughout life. In excitatory pyramidal neurons, plasticity is i...
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Article
PKCα integrates spatiotemporally distinct Ca2+ and autocrine BDNF signaling to facilitate synaptic plasticity
The protein kinase C (PKC) enzymes have long been established as critical for synaptic plasticity. However, it is unknown whether Ca2+-dependent PKC isozymes are activated in dendritic spines during plasticity an...
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Article
Erratum: An optical probe of synaptic plasticity
Nat. Biotechnol. 35, 26–27 (2017); published online 10 January 2017; corrected after print 30 March 2017 In the version of this article initially published, reference 1 was missing the author's first initial. ...
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Article
Precise small-molecule recognition of a toxic CUG RNA repeat expansion
Dimeric bis-benzimidazole compounds that bind selectively to toxic expanded r(CUG) RNA repeat sequences were identified and used as a scaffold for covalent modification, site-specific cleavage and on-target assem...
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Article
An optical probe of synaptic plasticity
An endogenous protein in the mouse brain is inactivated using a light-sensitive antibody construct.
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Article
Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins
Two red fluorescent proteins with long Stokes shift enable simultaneous multicolor 2p imaging. CyRFP1 is well-suited for 2p structural imaging, and FRET sensors made with mCyRFP1 and mMaroon1enable multicolor ...
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Article
Autocrine BDNF–TrkB signalling within a single dendritic spine
Live fluorescent imaging of murine hippocampal slices shows that NMDAR-dependent glutamate signalling leads to postsynaptic BDNF release, with associated signalling of its receptor, TrkB, on the same dendritic...
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Article
Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity
The three small GTPases Rac1, RhoA and Cdc42 are differentially involved in structural long-term potentiation of rodent dendritic spines, simultaneously ensuring signal specificity and also priming the system ...
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Article
A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo
In vivo imaging is facilitated by a bright, cyan-excitable orange fluorescent protein that is the basis of an improved bioluminescent protein.
