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  1. Article

    Open Access

    Iterative screening methodology enables isolation of strains with improved properties for a FACS-based screen and increased L-DOPA production

    Optimizing microbial hosts for the large-scale production of valuable metabolites often requires multiple mutations and modifications to the host’s genome. We describe a three-round screen for increased L-DOPA...

    Judy Savitskaya, Ryan J. Protzko, Francesca-Zhoufan Li, Adam P. Arkin in Scientific Reports (2019)

  2. Article

    Open Access

    Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste

    Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished...

    Ryan J. Protzko, Luke N. Latimer, Ze Martinho, Elise de Reus in Nature Communications (2018)

  3. Article

    Open Access

    Correction to: Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes

    The authors noticed an accidental calculation error existing in Fig. 4C of this article [1]. The given velocities of the GAT-1 transporter (in this figure only) were mistakenly listed 50-fold too high. Based on t...

    J. Philipp Benz, Ryan J. Protzko, Jonas M. S. Andrich in Biotechnology for Biofuels (2017)

  4. Article

    Open Access

    Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes

    Pectin-rich agricultural wastes potentially represent favorable feedstocks for the sustainable production of alternative energy and bio-products. Their efficient utilization requires the conversion of all majo...

    J Philipp Benz, Ryan J Protzko, Jonas MS Andrich in Biotechnology for Biofuels (2014)