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Open AccessCorrelating sugar transporter expression and activities to identify transporters for an orphan sugar substrate
Filamentous fungi like Neurospora crassa are able to take up and metabolize important sugars present, for example, in agricultural and human food wastes. However, only a fraction of all putative sugar transporter...
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Open AccessUnveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa
Classical fungal mutant strains obtained by mutagenesis have helped to elucidate fundamental metabolic pathways in the past. In the filamentous fungus Neurospora crassa, the gluc-1 strain was isolated long ago an...
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Open AccessEvaluation of Trichoderma isolates as biocontrol measure against Claviceps purpurea
Claviceps purpurea causes ergot, a floral disease of major cereal crops, such as winter rye and winter triticale. The dark sclerotia formed by C. purpurea contain numerous toxic ergot alkaloids, which pose a majo...
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Open AccessPress water from the mechanical drying of Douglas-fir wood chips has multiple beneficial effects on lignocellulolytic fungi
The mechanical drying of wood chips is an innovative method that improves the heating value of sawmill by-products in an energy-efficient continuous process. The liquid that comes out of the wood chips as pres...
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Open AccessVolatile organic compound patterns predict fungal trophic mode and lifestyle
Fungi produce a wide variety of volatile organic compounds (VOCs), which play central roles in the initiation and regulation of fungal interactions. Here we introduce a global overview of fungal VOC patterns a...
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Open AccessEngineering cofactor supply and NADH-dependent d-galacturonic acid reductases for redox-balanced production of l-galactonate in Saccharomyces cerevisiae
d-Galacturonic acid (GalA) is the major constituent of pectin-rich biomass, an abundant and underutilized agricultural byproduct. By one reductive step catalyzed by GalA reductases, GalA is converted to the pol.....
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Open AccessComparative evaluation of Aspergillus niger strains for endogenous pectin-depolymerization capacity and suitability for d-galacturonic acid production
Pectinaceous agricultural residues rich in d-galacturonic acid (d-GalA), such as sugar beet pulp, are considered as promising feedstocks for waste-to-value conversions. Aspergillus niger is known for its strong p...
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Open AccessGrowing a circular economy with fungal biotechnology: a white paper
Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnolog...
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Spotlight on fungal pectin utilization—from phytopathogenicity to molecular recognition and industrial applications
Pectin is a complex polysaccharide with d-galacturonic acid as its main component that predominantly accumulates in the middle lamella of the plant cell wall. Integrity and depolymerization of pectic structures h...
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Open AccessEngineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste
Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished...
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Open AccessGenome Sequencing and analyses of Two Marine Fungi from the North Sea Unraveled a Plethora of Novel Biosynthetic Gene Clusters
Marine Fungi are potent secondary metabolite producers. However, limited genetic information are available their biosynthetic gene clusters (BGCs) and their biotechnological applications. To overcome this lack...
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Open AccessCorrection to: Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes
The authors noticed an accidental calculation error existing in Fig. 4C of this article [1]. The given velocities of the GAT-1 transporter (in this figure only) were mistakenly listed 50-fold too high. Based on t...
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HPAEC-PAD for oligosaccharide analysis—novel insights into analyte sensitivity and response stability
The rising importance of accurately detecting oligosaccharides in biomass hydrolyzates or as ingredients in food, such as in beverages and infant milk products, demands for the availability of tools to sensiti...
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Open AccessComparing the physiochemical parameters of three celluloses reveals new insights into substrate suitability for fungal enzyme production
The industrial applications of cellulases are mostly limited by the costs associated with their production. Optimized production pathways are therefore desirable. Based on their enzyme inducing capacity, cellu...
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Open AccessNetwork reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa
Plant biomass degradation by fungal-derived enzymes is rapidly expanding in economic importance as a clean and efficient source for biofuels. The ability to rationally engineer filamentous fungi would facilita...
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Open AccessThe transcription factor PDR-1 is a multi-functional regulator and key component of pectin deconstruction and catabolism in Neurospora crassa
Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role fo...
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The Renaissance of Neurospora crassa: How a Classical Model System is Used for Applied Research
One major focus in fungal biotechnological research is the elucidation of plant biomass degradation. Filamentous fungi have the capacity to produce large amounts of enzymes for lignocellulose deconstruction th...
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Open AccessIdentification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes
Pectin-rich agricultural wastes potentially represent favorable feedstocks for the sustainable production of alternative energy and bio-products. Their efficient utilization requires the conversion of all majo...
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Open AccessTic20 forms a channel independent of Tic110 in chloroplasts
The Tic complex (Translocon at the inner envelope membrane of chloroplasts) mediates the translocation of nuclear encoded chloroplast proteins across the inner envelope membrane. Tic110 forms one prominent protei...
