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Article
Open AccessA genome-wide association study reveals novel loci and candidate genes associated with plant height variation in Medicago sativa
Plant height (PH) is an important agronomic trait influenced by a complex genetic network. However, the genetic basis for the variation in PH in Medicago sativa remains largely unknown. In this study, a comprehen...
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Article
Open AccessOverexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula
Elongation factor 1 A (EF1A), an essential regulator for protein synthesis, has been reported to participate in abiotic stress responses and environmental adaption in plants. However, the role of EF1A in abiotic ...
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Article
Open AccessGenome-wide identification, phylogeny and expression analysis of the SPL gene family and its important role in salt stress in Medicago sativa L.
SQUAMOSA promoter-binding protein-like (SPL) transcription factors are widely present in plants and are involved in signal transduction, the stress response and development. The SPL gene family has been character...
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Article
Open AccessiTRAQ-based comparative proteomic analysis of differences in the protein profiles of stems and leaves from two alfalfa genotypes
To explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype a...
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Article
Open AccessGenetic map** of leaf-related traits in autotetraploid alfalfa (Medicago sativa L.)
Understanding the genetic architecture of leaf-related traits is important for improving alfalfa yield. Leaf size has a great influence on the protein content and yield for alfalfa. In this study, a low-yieldi...
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Article
Proteomic Analysis of Shoot Tips from Two Alfalfa Cultivars with Different Florescence
Flowering is an indispensable biological process for the complete life cycle of angiosperms, crucial to the regeneration of plants and the continuation of species. In this work, a proteomic approach was applie...
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Article
Open AccessHigh-density linkage map construction and map** QTL for yield and yield components in autotetraploid alfalfa using RAD-seq
Alfalfa (Medicago sativa L.) is an important forage crop grown worldwide. Alfalfa is called the “queen of forage crops” due to its high forage yield and nutritional characteristics. The aim of this study was to u...
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Article
A Novel miRNA Sponge Form Efficiently Inhibits the Activity of miR393 and Enhances the Salt Tolerance and ABA Insensitivity in Arabidopsis thaliana
MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level in eukaryotes by directing target mRNAs for degradation or by repressing translation. MicroRNAs are involved in many processes, inc...
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Article
Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.)
Heat shock proteins (HSPs) are a ubiquitously expressed class of protective proteins that play a key role in plant response to stressful conditions. This study aimed to characterize and investigate the functio...
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Article
Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula
Salt stress is one of the most significant adverse abiotic factors, causing crop failure worldwide. So far, a number of salt stress-induced genes, and genes improving salt tolerance have been characterized in ...
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Article
Molecular cloning and functional analysis of a salt-induced gene encoding an RNA-binding protein in alfalfa
Based on the sequence of an expressed sequence tag of alfalfa (Medicago sativa L. cv. Zhongmu-1), a full-length sequence of 1,551 nucleotides was isolated using RACE-PCR techniques. This gene (MsRBP) was predicte...
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Article
Overexpression of a novel salt stress-induced glycine-rich protein gene from alfalfa causes salt and ABA sensitivity in Arabidopsis
We cloned a novel salt stress-induced glycine-rich protein gene ( MsGRP ) from alfalfa. Its overexpression retards seed germination and seedling growth of tr...