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Article
Open AccessCloning of sft-4 and its influence on vitality and virulence of pine wood nematode, Bursaphelenchus xylophilus
In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease, pine wood nematode (PWN, Bursaphelenchus xylophilus), after treatment with the nematicide fomepizole, Surfe...
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Article
Synergistic antibacterial performance of rosemarinic acid - graphene oxide in electrospun polylactic acid membranes for air filtration
Polylactic acid (PLA) is a kind of widely used, biodegradable, biomass polymer material, and the PLA fibrous material with high antibacterial efficiency has attracted more and more attention. In this article, ...
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Article
Nematicidal phytochemicals against pine wood nematode, Bursaphelenchus xylophilus (nematoda: aphelenchoididae)
AS the major causative agent of pine wilt disease, pine wood nematode, Bursaphelenchus xylophilus can result in rapid and massive deaths of pine forest, and even severe economy burdens globally. Botanical nematic...
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Article
Rapid Detection of the Bursaphelenchus Xylophilus by Denaturation Bubble-mediated Strand Exchange Amplification
Bursaphelenchus xylophilus (B. xylophilus) is one of the most important causal agents of infectious diseases in forest pathology. Obviously, the rapid detection of B. xylophilus is an urgent need for its preventi...
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Article
Open AccessEvent-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods
The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...
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Article
Overexpression and activities of 1-Cys peroxiredoxin from Pseudomonas fluorescens GcM5-1A carried by pine wood nematode
Peroxiredoxins (Prxs) are enzymatic antioxidants widely distributed in biological kingdoms, which constitute a family of heme-free peroxidases that reduce alkyl hydroperoxides and hydrogen peroxide. In this pa...
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Article
Heme oxygenase-1 silencing increases the sensitivity of human osteosarcoma MG63 cells to arsenic trioxide
Arsenic trioxide (ATO) has been successfully used to treat leukemia and some solid malignant tumors. Our previous study regarding the effects of ATO on mesenchymal-derived human osteosarcoma MG63 cells showed ...
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Article
Effects of Pseudomonas fluorescens flagellin on physiological and biochemical characteristics in the suspension cells of Pinus thunbergii
In order to investigate the effects of flagellin secreted by bacteria associated with pine wood nematode (Bursaphelenchus xylophilus) on pine cells, suspension cells of Pinus thunbergii were treated with flagelli...
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Article
Silencing SATB1 inhibits proliferation of human osteosarcoma U2OS cells
It has been shown that over-expression of Special AT-rich binding protein 1 (SATB1) in breast cancer predicts a poor prognosis. This study was aimed at investigating the effects of silencing SATB1 on mesenchym...
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Article
Arsenic trioxide induces apoptosis of p53 null osteosarcoma MG63 cells through the inhibition of catalase
This study is aimed at investigating the effect of arsenic trioxide (ATO) on p53 null human osteosarcoma MG63 cells and the mechanisms underlying the effect. Apoptotic cells were detected by flow cytometry wit...
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Article
Arsenic Induces Apoptosis of Human Umbilical Vein Endothelial Cells Through Mitochondrial Pathways
To clarify the molecular mechanisms through which arsenic causes injuries to blood vessels, we analyzed the effects of sodium arsenite (NaAsO2) on the apoptosis of human umbilical vein endothelial cells (HUVECs),...
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Article
Cloning and expression of a toxin gene from Pseudomonas fluorescens GcM5-1A
Pseudomonas fluorescens GcM5-1A was isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, obtained from wilted Japanese black pine, Pinus thumbergii, in China. In this paper, a genomic library o...
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Article
Rosmarinic Acid Inhibits 6-OHDA-induced Neurotoxicity by Anti-oxidation in MES23.5 Cells
Rosmarinic acid (RA) is a naturally occurring polyphenolic compound. It is found in several herbs in the Lamiaceae family, such as Perilla frutescens. RA has been reported to exert anti-oxidative effects on rat e...
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Article
On the Role of the General Transcription Factor Sp1 in the Activation and Repression of Diverse Mammalian Oxidative Phosphorylation Genes*
To gain insight into the role of the general transcription factor,Sp1, in the expression of nuclear genes involved in mitochondrial biogenesis,we investigated Sp1 activation of the adenine nucleotide transloca...
