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  1. No Access

    Chapter

    In Vitro Culture and Genetic Transformation in Mango

    Mango is one of the world’s most important fruit crops and is widely grown in the tropics and subtropics. Despite its importance in local economies and as an increasingly important export crop, very little pro...

    César Petri, Richard E. Litz, Sanjay K. Singh, José I. Hormaza in The Mango Genome (2021)

  2. No Access

    Article

    Agrobacterium tumefaciens-mediated transformation of 'Brewster' ('Chen Tze') litchi (Litchi chinensis Sonn.) with the PISTILLATA cDNA in antisense

    In order to address the need for production of parthenocarpic fruits, embryogenic cultures of 'Brewster' ('Chen Tze') litchi derived from leaves of a mature tree were transformed with the PISTILLATA (PI) cDNA in ...

    Guillermo Padilla, José A. Pérez… in In Vitro Cellular & Developmental Biology … (2013)

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    Article

    Recovery of avocado (Persea americana Mill.) plants transformed with the antifungal plant defensin gene PDF1.2

    Embryogenic avocado cultures derived from ‘Hass’ protoplasts were genetically transformed with the plant defensin gene (pdf1.2) driven by the CaMV 35S promoter in pGPTV with uidA as a reporter gene and bar, the g...

    Simon H. T. Raharjo, Witjaksono… in In Vitro Cellular & Developmental Biology … (2008)

  4. No Access

    Article

    Somatic embryogenesis and plant regeneration of litchi (Litchi chinensis Sonn.) from leaves of mature phase trees

    Embryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster’ litchi trees on B5 medium containing 400 mg l−1 glutamine, 200 mg l−1 casein hydrolysate, 30 g l−1 sucrose, 4.52 μM...

    Simon H. T. Raharjo, Richard E. Litz in Plant Cell, Tissue and Organ Culture (2007)

  5. No Access

    Article

    Recovery and Indexing of Avocado Plants (Persea Americana) from Embryogenic Nucellar Cultures of an Avocado Sunblotch Viroid-Infected Tree

    Avocado (Persea americana Mill.) plants were recovered from somatic embryos derived from the nucellus of an avocado sunblotch viroid (ASBVd)-infected tree (???Vero Beach??? SE2). Embryogen...

    Isidro E. Suarez, Raymond A. Schnell, David N. Kuhn… in Plant Cell, Tissue and Organ Culture (2006)

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    Article

    Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)

    The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro. The production of morphologically normal, bipolar somatic...

    Simon H. T. Raharjo, Richard E. Litz in Plant Cell, Tissue and Organ Culture (2005)

  7. No Access

    Article

    Micrografting of ASBVd-infected Avocado (Persea americana) plants

    Shoot tips (meristem plus 2–3 leaf primordia) from in vitro-germinated avocado seedlings of 2 ASBVd-infected cultivars were micrografted in vitro onto decapitated seedlings from 2 ASBVd-free cultivars, and plants...

    Isidro E. Suarez, Raymond A. Schnell, David N. Kuhn… in Plant Cell, Tissue and Organ Culture (2005)

  8. No Access

    Article

    Genetic transformation of perennial tropical fruits

    Genetic transformation provides the means for modifying single horticultural traits in perennial plant cultivars without altering their phenotype. This capability is particularly valuable for perennial plants ...

    Miguel A. Gómez-Lim, Richard E. Litz in In Vitro Cellular & Developmental Biology - Plant (2004)

  9. No Access

    Article

    Effect of Gamma Irradiation on Embryogenic Avocado Cultures and Somatic Embryo Development

    Embryogenic avocado cultures were exposed to ionizing irradiation in order to determine its effect on proliferation and subsequent somatic embryo development. The approximate PD50 as determined by linear regressi...

    Witjaksono, Richard E. Litz in Plant Cell, Tissue and Organ Culture (2004)

  10. No Access

    Article

    A biotechnology strategy for medium- and long-term conservation of cycads

    It has been possible to regenerate a few cycad species in vitro by somatic embryogenesis, either from zygotic embryos (Ceratozamia hildae, C. mexicana, Encephalartos cycadifolius,E. dyerianus, E. natalensis, Zami...

    Richard E. Litz, P. A. Moon, E. M. Benson, J. Stewart… in The Botanical Review (2004)

  11. No Access

    Article

    Effect of gelling agent on growth and development ofCeratozamia hildae somatic embryos

    Individual somatic proembryos ofCeratozamia hildae were exposed to media that differed only in gelling agent utilized. Five different gelling agents were compared in the first experiment: Bacto agar, Agargel, Gel...

    P. A. Moon, Richard E. Litz, Victor M. Chávez in The Botanical Review (2004)

  12. No Access

    Article

    Responses of embryogenic mango cultures and seedling bioassays to a partially purified phytotoxin produced by a mango leaf isolate of Colletotrichum gloeosporioides penz

    Colletotrichum gloeosporioides Penz., the causal agent of mango anthracnose, produces a phytotoxin in vitro. The partially purified phytotoxin, presumably colletotrichin, caused anthracno...

    S. Jayasankar, Richard E. Litz… in In Vitro Cellular & Developmental Biology … (1999)

  13. No Access

    Article

    Organogenesis from megagametophyte and zygotic embryo explants of the gymnosperm Dioon edule Lindley (Zamiaceae, Cycadales)

    Organogenic cultures were induced from zygotic embryo and megagametophyte explants of the Central American cycad species, Dioon edule. Plant growth medium consisted of B5 major salts, Murashige and Skoog minor sa...

    Victor M. Chavez, Richard E. Litz in Plant Cell, Tissue and Organ Culture (1999)

  14. No Access

    Article

    Avocado shoot culture, plantlet development and net CO2 assimilation in an ambient and CO2 enhanced environment

    The proliferation and survival of avocado nodal cultures of juvenile origin were affected by the form and concentration of nitrogen. Optimum growth was achieved on modified Murashige and Skoog medium containin...

    Witjaksono, Bruce A. Schaffer… in In Vitro Cellular & Developmental Biology … (1999)

  15. No Access

    Article

    Embryogenic mango cultures selected for resistance toColletotrichum gloeosporioides culture filtrate show variation in random amplified polymorphic DNA (RAPD) markers

    Genomic DNA isolated from embryogenic cultures of two mango cultivars, ‘Hindi’ and ‘Carabao,’ that had been selected for resistance to the culture filtrate ofColletotrichum gloeosporioides, was analyzed using Ran...

    S. Jayasankar, Richard E. Litz… in In Vitro Cellular & Developmental Biology … (1998)

  16. No Access

    Article

    Effects of 1-aminocyclopropane-1-carboxylic acid, aminoethoxyvinylglycine, methylglyoxal bis-(guanylhydrazone) and dicyclohexylammonium sulfate on induction of embryogenic competence of mango nucellar explants

    The effects of aminoethoxyvinylglycine (AVG),1-aminocyclopropane- 1 -carboxylic acid (ACC), dicycyclohexylammonium sulfate (DCHA) and methylglyoxal bis-(guanylhydrazone) (MGBG) on induction of embryogenic comp...

    Richard E. Litz, C. Yurgalevitch in Plant Cell, Tissue and Organ Culture (1997)

  17. No Access

    Article

    Effect of abscisic acid, osmolarity and partial desiccation on the development of recalcitrant mango somatic embryos

    Inhibition of mango somatic embryo growth was inducedin vitro by treatments for 4 or more weeks with abscisic acid (0–100 μM ABA) with and without high osmolarity provided by mannitol (0–10%). High osmolarity and...

    Fernando Pliego-Alfaro, Mary Joy R. Monsalud… in Plant Cell, Tissue and Organ Culture (1996)

  18. No Access

    Article

    Effect of abscisic acid, osmolarity and temperature onin vitro development of recalcitrant mango nucellar embryos

    Development of cotyledonary-stage nucellar embryos of mango was arrestedin vitro by exposure to 750–1750 μM ABA. The enlargement and germination of nucellar embryos was inhibited for as long as 4 weeks after subc...

    Fernando Pliego-Alfaro, Richard E. Litz… in Plant Cell, Tissue and Organ Culture (1996)

  19. No Access

    Article

    Control of hyperhydricity of mango somatic embryos

    Hyperhydricity of immature somatic embryos has been a limiting factor for the development of highly embryogenic suspension cultures of many important mango cultivars. Reversion of hyperhydricity was achieved i...

    Mary-Joy Monsalud, Helena Mathews, Richard E. Litz… in Plant Cell, Tissue and Organ Culture (1995)

  20. No Access

    Article

    Somatic embryogenesis from leaf callus derived from mature trees of the cycad Ceratozamia hildae (Gymnospermae)

    A friable and transient embryogenic callus was initiated from pinnae removed from leaves in new vegetative flushes of mature Ceratozamia hildae Landry & Wilson, a cycad. Somatic proembryos developed from the call...

    Richard E. Litz, Pamela A. Moon, Victor M. Chavez in Plant Cell, Tissue and Organ Culture (1995)

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