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  1. No Access

    Article

    Biochemical characterization of developmental stages of cycad somatic embryos

    The effect of two light intensities (25 μmol m−2s−1 and 50 μmol m−2s−1) on four developmental stages ofCeratozamia mexicana somatic embryos growing on semisolid plant growth medium at 25°C was measured. Growth pa...

    I. Vargas-Luna, G. Ortiz-Montiel, V. M. Chávez, R. E. Litz… in The Botanical Review (2004)

  2. No Access

    Article

    A biotechnology strategy for medium- and long-term conservation of cycads

    It has been possible to regenerate a few cycad species in vitro by somatic embryogenesis, either from zygotic embryos (Ceratozamia hildae, C. mexicana, Encephalartos cycadifolius,E. dyerianus, E. natalensis, Zami...

    Richard E. Litz, P. A. Moon, E. M. Benson, J. Stewart… in The Botanical Review (2004)

  3. No Access

    Article

    Effect of gelling agent on growth and development ofCeratozamia hildae somatic embryos

    Individual somatic proembryos ofCeratozamia hildae were exposed to media that differed only in gelling agent utilized. Five different gelling agents were compared in the first experiment: Bacto agar, Agargel, Gel...

    P. A. Moon, Richard E. Litz, Victor M. Chávez in The Botanical Review (2004)

  4. No Access

    Article

    Regeneration of Ceratozamia euryphyllidia (Cycadales, Gymnospermae) plants from embryogenic leaf cultures derived from mature-phase trees

    Embryogenic cultures were induced from pinnae removed from young leaf flushes of mature-phase trees of the endangered cycad species, Ceratozamia euryphyllidia. Induction media consisted of B5 major salts, Murash...

    V. M. Chavez, R. E. Litz, M. Monroy, P. A. Moon, A. M. Vovides in Plant Cell Reports (1998)

  5. No Access

    Article

    Induction of embryogenic mango cultures as affected by genotype, explanting, 2,4-D and embryogenic nurse culture

    The nucellus was removed from immature seeds of 4 mango genotypes, andcultured under different induction conditions. The mango genotypes includedpolyembryonic ‘Hindi’ and ‘Nam Doc Mai’ and monoembryonic ‘Lippe...

    R.E. Litz, R.C. Hendrix, P.A. Moon, V. M. Chavez in Plant Cell, Tissue and Organ Culture (1998)

  6. No Access

    Article

    Temporal effect of 2,4-D on induction of embryogenic nuclellar cultures and somatic embryo development of ‘Carabao’ mango

    Embryogenic nucellar cultures were established on B5 major salts, MS minor salts and organics, 400 mg/l−1 glutamine, 60 g/l−1 sucrose, 2 g/l−1 gellan gum, and 4.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D). There ...

    B. L. Lad, S. Jayasankar, F. Pliego-Alfaro… in In Vitro Cellular & Developmental Biology … (1997)

  7. No Access

    Article

    Somatic embryogenesis from leaf callus of mature plants of the gymnospermCeratozamia mexicana var. robusta (Miq.) dyer (Cycadales)

    Embryogenic callus was induced from explanted pinnae of newly emerged leaves of mature plants ofCeratozamia mexicana var. Robusta (Gymnospermae, Cycadales) on a modified B5 formulation with 1 mg·liter−1 kinetin a...

    V. M. Chavez, R. E. Litz, P. A. Moon, K. Norstog in In Vitro – Plant (1992)