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  1. Article

    Open Access

    Biodegradation of p-nitrophenol by engineered strain

    p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove .....

    **g Xu, Bo Wang, Wen-hui Zhang, Fu-Jian Zhang, Yong-dong Deng, Yu Wang… in AMB Express (2021)

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  2. No Access

    Article

    VvMYBA6 in the promotion of anthocyanin biosynthesis and salt tolerance in transgenic Arabidopsis

    Transcription factor MYB is a critical factor involved in anthocyanin biosynthesis and abiotic stress resistance of plants. This paper reports the functions of codon-optimized transcription factor VvMYBA6 express...

    Miao Sun, **n-**n Feng, Jian-Jie Gao, Ri-He Peng… in Plant Biotechnology Reports (2017)

  3. No Access

    Article

    Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice

    The ADI1 Arabidopsis plants enhanced tolerance and degradation efficiency to naphthalene and had great potential for phytoremediation of na...

    **ao-Yan Fu, Bo Zhu, Hong-Juan Han, Wei Zhao, Yong-Sheng Tian… in Plant Cell Reports (2016)

  4. No Access

    Article

    Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae

    2,4,6-Trinitrotoluene (TNT) is released into natural environment from demilitarization facilities, manufacturing, and explosive remnants of war; this compound is one of the most recalcitrant explosives. TNT co...

    Shuang-Hong You, Bo Zhu, Hong-Juan Han, Bo Wang, Ri-He Peng… in Plant Biotechnology Reports (2015)

  5. No Access

    Article

    Improved glyphosate resistance of 5-enolpyruvylshikimate-3-phosphate synthase from Vitis vinifera in transgenic Arabidopsis and rice by DNA shuffling

    To date, only AroA variant derived from Agrobacterium tumefaciens CP4 has been used to generate the commercial glyphosate-resistant crops currently available in the market. This single source of the EPSPS gene ma...

    Yong-Sheng Tian, **g Xu, **ao-Juan **ng, Wei Zhao, **ao-Yan Fu… in Molecular Breeding (2015)

  6. No Access

    Article

    Complementary screening, identification and application of a novel class II 5-enopyruvylshikimate-3-phosphate synthase from Bacillus cereus

    A novel aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase from Bacillus cereus was identified and overexpressed by genomic library construction and complementary screening. The enzyme was then purifi...

    Yong-Sheng Tian, **g Xu, **g Han, Wei Zhao… in World Journal of Microbiology and Biotechn… (2013)

  7. No Access

    Article

    Phytoremediation of triphenylmethane dyes by overexpressing a Citrobacter sp. triphenylmethane reductase in transgenic Arabidopsis

    Triphenylmethane dyes are extensively utilized in textile industries, medicinal products, biological stains, and food processing industries, etc. They are generally considered as xenobiotic compounds, which ar...

    **ao-Yan Fu, Wei Zhao, Ai-Sheng **ong… in Applied Microbiology and Biotechnology (2013)

  8. No Access

    Article

    Characterization of a thermostable β-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana

    TmGUSI, a gene identical to that encoding a thermostable β-glucuronidase in the hyperthermophilic anaerobe Thermotoga maritima, has been synthesized using a PCR-based two-step DNA synthesis and c...

    **g Xu, Yong-Sheng Tian, Ri-He Peng, Bo Zhu… in Applied Microbiology and Biotechnology (2012)

  9. No Access

    Article

    Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis

    Although a large number of AroA enzymes (5-enopyruvylshikimate-3-phosphate synthase [EPSPS]) have been identified, cloned and tested for glyphosate resistance, only AroA variants derived from Agrobacterium tumefa...

    Yong-Sheng Tian, **g Xu, Ai-Sheng **ong… in Applied Microbiology and Biotechnology (2012)

  10. No Access

    Article

    Characterization of a Glucose-, Xylose-, Sucrose-, and d-Galactose-Stimulated β-Glucosidase from the Alkalophilic Bacterium Bacillus halodurans C-125

    The gene (Bhbgl) encoding a β-glucosidase from the alkalophilic bacterium Bacillus halodurans C-125 was synthesized chemically via the PCR-based two-step DNA synthesis (PTDS) method and expressed in Escherichia c...

    Hu Xu, Ai-Sheng **ong, Wei Zhao, Yong-Sheng Tian, Ri-He Peng… in Current Microbiology (2011)

  11. No Access

    Article

    Identification and Analysis of Polychlorinated Biphenyls (PCBs)-Biodegrading Bacterial Strains in Shanghai

    As one of China’s great metropolises, Shanghai is vulnerable to various forms of industrial and agricultural contamination associated with its development. Polychlorinated biphenyls (PCBs) are man-made chemica...

    Jian-Jun Shuai, Yong-Sheng Tian, Quan-Hong Yao, Ri-He Peng… in Current Microbiology (2010)

  12. No Access

    Article

    Mutations in two amino acids in phyI1s from Aspergillus niger 113 improve its phytase activity

    We applied in vitro mutagenesis and colony screening, using the wild type phyI1s gene from Aspergillus niger 113 as the template, and obtained two mutant phyI1s (gene products) after one round of screening. The t...

    Yong-Sheng Tian, Ri-He Peng, **g Xu… in World Journal of Microbiology and Biotechn… (2010)

  13. No Access

    Article

    Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity

    We performed directed evolution on a chemically synthesized 1,533-bp recombinant beta-galactosidase gene from Pyrococcus woesei. More than 200,000 variant colonies in each round of directed evolution were screene...

    Ai-Sheng **ong, Ri-He Peng, **g Zhuang, **an Li… in Applied Microbiology and Biotechnology (2007)

  14. No Access

    Article

    Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza Sativa L.

    Using a yeast one-hybrid method, a transcription factor, OsAP25, which interacts specifically with a GCC box was isolated from rice. The OsAP25 protein contained a conserved ethylene-responsive element binding...

    **ao-Yan Fu, Zhen Zhang, Ri-He Peng, Ai-Sheng **ong, **-Ge Liu… in Biotechnology Letters (2007)

  15. No Access

    Article

    Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L.

    A previous study had indicated that the transcription factors DREB/CBF (DRE-binding protein/C-repeat binding factor) play important roles in the expression of many stress inducible genes under cold-temperature...

    Qiu-lin Qin, **-ge Liu, Zhen Zhang, Ri-he Peng, Ai-sheng **ong… in Molecular Breeding (2007)

  16. No Access

    Article

    High efficiency and throughput system in directed evolution in vitro of reporter gene

    In vitro directed evolution, especially with DNA shuffling, is a powerful means in biological studies of protein structure and function, and consequently for industrial applications. Escherichia coli β-glucuronid...

    Ai-Sheng **ong, Ri-He Peng, **-Ge Liu… in Applied Microbiology and Biotechnology (2007)

  17. No Access

    Article

    Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.

    A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L. by a yeast one-hybrid method. Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a con...

    **-Ge Liu, Zhen Zhang, Qiu-Lin Qin, Ri-He Peng, Ai-Sheng **ong… in Biotechnology Letters (2007)

  18. No Access

    Article

    A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis

    A simple, two-step efficient method to perform multiple-site mutagenesis of a gene from bacterial genome was developed. The method was named polyacrylamide gel electrophoresis (PAGE)-mediated overlap extension...

    Ri-He Peng, Ai-Sheng **ong, Quan-Hong Yao in Applied Microbiology and Biotechnology (2006)

  19. No Access

    Article

    High level expression of a synthetic gene encoding Peniophora lycii phytase in methylotrophic yeast Pichia pastoris

    Phytase is widespread in nature. It has been used as a cereal feed additive that can enhance the phosphorus and mineral absorption in monogastric animals to reduce the level of phosphorus output in manure. Phy...

    Ai-Sheng **ong, Quan-Hong Yao, Ri-He Peng… in Applied Microbiology and Biotechnology (2006)

  20. No Access

    Article

    Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

    Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus ph...

    Ri-He Peng, Quan-Hong Yao, Ai-Sheng **ong, Zong-Ming Cheng, Yi Li in Plant Cell Reports (2006)

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