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Article
Open AccessMetabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose
Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-...
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Article
Open AccessBiodegradation of p-nitrophenol by engineered strain
p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove .....
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Article
Transgenic Arabidopsis Plants Expressing Grape Glutathione S-Transferase Gene (VvGSTF13) Show Enhanced Tolerance to Abiotic Stress
Although glutathione S-transferase (GST, EC 2.5.1.18) is thought to play important roles in abiotic stress, limited information is available regarding the function of its gene in grapes. In this study, a GST g...
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Article
Ectopic expression of sorbitol-6-phosphate 2-dehydrogenase gene from Haloarcula marismortui enhances salt tolerance in transgenic Arabidopsis thaliana
d-Sorbitol-6-phosphate 2-dehydrogenase (S6PDH, E.C. 1.1.1.140) catalyzes the NADH-dependent conversion of d-fructose 6-phosphate (F6P) to d-sorbitol 6-phosphate (S6P). In this work, recombination and characteriza...
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Article
VvMYBA6 in the promotion of anthocyanin biosynthesis and salt tolerance in transgenic Arabidopsis
Transcription factor MYB is a critical factor involved in anthocyanin biosynthesis and abiotic stress resistance of plants. This paper reports the functions of codon-optimized transcription factor VvMYBA6 express...
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Article
Enhancement of phenol stress tolerance in transgenic Arabidopsis plants overexpressing glutathione S-transferase
Plant glutathione S-transferases (GSTs) are important for protecting plants against oxidative damage. We studied the function of a glutathione S-transferase family protein in Arabidopsis, AtGSTF2. Our results ind...
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Article
Open AccessDisulfide isomerase-like protein AtPDIL1–2 is a good candidate for trichlorophenol phytodetoxification
Trichlorophenol (TCP) is a widely used and persistent environmentally toxic compound that poses a carcinogenic risk to humans. Phytoremediation is a proficient cleanup technology for organic pollutants. In thi...
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Article
Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice
The ADI1 Arabidopsis plants enhanced tolerance and degradation efficiency to naphthalene and had great potential for phytoremediation of na...
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Article
Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae
2,4,6-Trinitrotoluene (TNT) is released into natural environment from demilitarization facilities, manufacturing, and explosive remnants of war; this compound is one of the most recalcitrant explosives. TNT co...
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Article
Open AccessIdentification of a phosphinothricin-resistant mutant of rice glutamine synthetase using DNA shuffling
To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused ...
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Article
Improving Rice Ability to Degrade Different Polycyclic Aromatic Hydrocarbons Through Multigene Transformation of a Hybrid Dioxygenase System
The widespread presence and potential harm conferred by polycyclic aromatic hydrocarbons (PAHs) to various organisms have raised increased research interest on these pollutants. Plants lack of efficient PAH de...
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Article
Gene expression profile of Arabidopsis under sodium bisulfite treatment by oligo-microarray analysis
Low concentrations of sodium bisulfite (NaHSO3) can enhance some plant photosynthetic efficiency. To investigate the growth process of Arabidopsis seedlings in response to NaHSO3 treatment and to obtain a better ...
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Article
Improved glyphosate resistance of 5-enolpyruvylshikimate-3-phosphate synthase from Vitis vinifera in transgenic Arabidopsis and rice by DNA shuffling
To date, only AroA variant derived from Agrobacterium tumefaciens CP4 has been used to generate the commercial glyphosate-resistant crops currently available in the market. This single source of the EPSPS gene ma...
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Article
Improvement of the ability to produce spinosad in Saccharopolyspora spinosa through the acquisition of drug resistance and genome shuffling
Spinosyns, a secondary metabolite from the fermentation of Saccharopolyspora spinosa, exhibits evident insecticidal activity. The most active components of the spinosyns family are spinosyns A and D, which are ma...
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Article
Gene expression profiles of arabidopsis under the stress of methyl viologen: a microarray analysis
Methyl viologen (MV) is the main ingredient of Paraquat. It is little known about how plants respond to this compound. To understand the mode of MV action and molecular mechanism of plant response, we perform...
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Article
Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Proteus mirabilis was identified and isolated. Furthermore, the t...
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Article
A Rice OsAP23, Functioning as an AP2/ERF Transcription Factor, Reduces Salt Tolerance in Transgenic Arabidopsis
The AP2/ERF transcription factors of higher plants regulate transcriptional networks that control development and responses to biotic and abiotic stresses. In this study, the rice OsAP23 gene, which encodes an AP...
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Article
An improved integrative transformation system for Pichia pastoris with DNA-polyethylenimine-dextran sulfate nanoparticles
Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it ...
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Article
Complementary screening, identification and application of a novel class II 5-enopyruvylshikimate-3-phosphate synthase from Bacillus cereus
A novel aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase from Bacillus cereus was identified and overexpressed by genomic library construction and complementary screening. The enzyme was then purifi...
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Article
Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis
One of the most rapid and effective defensive mechanisms plants have for protecting themselves, from a variety of biotic and abiotic stresses, is the regulation of plant signal transcription factors. AP2/ERF f...
