-
Chapter
Analyse der epigenetischen Modifikationen
Die wichtigste epigenetische Modifikation der DNA ist die Methylierung von Cytosin am C5-Atom zu 5-Methylcytosin 5mC (◘ Abb. 35.1). 5-Methylcytosin kann zu 5-Hydroxymethylcytosin 5hmC, 5-Formylcytosin 5fC und 5-C...
-
Article
Open AccessIdentification of 5 novel genes methylated in breast and other epithelial cancers
There are several high throughput approaches to identify methylated genes in cancer. We utilized one such recently developed approach, MIRA (methylated-CpG island recovery assay) combined with CpG island array...
-
Article
Open AccessEpigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma
The ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) gene involved in the regulation of cellular ubiquitin levels plays an important role in different cellular processes including cell growth and differentiatio...
-
Article
Cultivated keratinocytes express N-methyl-d-aspartate receptors of the NMDAR2D type
N-methyl-d-aspartate receptors (NMDAR) can regulate the intracellular calcium concentration of keratinocytes (KC) and seem to be important for their growth and differentiation. The objective of this study was to ...
-
Article
Control of microtubule stability by the RASSF1A tumor suppressor
The RAS association domain family 1A (RASSF1A) gene is silenced by DNA methylation in over 50% of all solid tumors of different histological types. However, the biochemical function of the RASSF1A protein is unkn...
-
Article
Frequent RASSF1A promoter hypermethylation and K-ras mutations in pancreatic carcinoma
Recently, we have characterized the Ras association domain family 1A gene (RASSF1A) at the segment 3p21.3, which is frequently lost in variety of human cancers and epigenetically inactivated in many types of prim...
-
Article
Frequent epigenetic inactivation of the RASSF1A gene in hepatocellular carcinoma
Aberrant promoter methylation is a fundamental mechanism of inactivation of tumor suppressor genes in cancer. The Ras association domain family 1A gene (RASSF1A) is frequently epigenetically silenced in several t...
-
Article
Frequent hypermethylation of the RASSF1A gene in prostate cancer
Recently, we have cloned and characterized the Ras association domain family 1A gene (RASSF1A) at 3p21.3, from which loss of genetic material is one of the most frequent events in several types of human solid tum...
-
Article
RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1
RASSF1A, one of the two major isoforms of the putative tumor suppressor gene RASSF1, located at 3p21.3, is inactivated in a variety of human cancers including lung, breast, bladder and renal cell carcinomas. We h...
-
Article
Erratum: The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1
Correction to: Oncogene (2002) 21, 1381–1390. DOI: 10.1038/sj.onc.1205192 The authors wish to apologise for an error found in the discussion section. A revised version of the text is given below: Moreover, alt...
-
Article
The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1
Nore and RASSF1A are noncatalytic proteins that share 50% identity over their carboxyterminal 300 AA, a segment that encompasses a putative Ras-Rap association (RA) domain. RASSF1 is expressed as several splic...
-
Article
The CpG island of the novel tumor suppressor gene RASSF1A is intensely methylated in primary small cell lung carcinomas
Loss of heterozygosity at 3p21.3 occurs in more than 90% of small cell lung carcinomas (SCLCs). The Ras association domain family 1 (RASSF1) gene cloned from the lung tumor suppressor locus 3p21.3 consists of two...
-
Article
Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3
Allelic loss at the short arm of chromosome 3 is one of the most common and earliest events in the pathogenesis of lung cancer, and is observed in more than 90% of small-cell lung cancers (SCLCs) and in 50–80%...
-
Protocol
Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
Several types of DNA lesions are formed on irradiation of cells with ultraviolet (UV) light (1,2). The two most frequent lesions are the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine (6-4) pyrimidone ph...
-
Protocol
In Vivo Map** of Nucleosomes Using Psoralen-DNA Crosslinking and Primer Extension
In the nucleus of eukaryotic cells, DNA is packaged into a nucleoprotein complex known as chromatin (1). This complex provides the compaction and structural organization of the DNA for processes such as replicati...
