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Chapter
Photosynthetic Light Reactions in Diatoms. II. The Dynamic Regulation of the Various Light Reactions
Photosynthesis in diatoms is performed using the same basic modules as cyanobacteria and plants. It can be regulated on multiple levels depending on the environmental cues, allowing diatoms to adjust their pho...
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Chapter
Photosynthetic Light Reactions in Diatoms. I. The Lipids and Light-Harvesting Complexes of the Thylakoid Membrane
Light harvesting and photochemistry is performed by photosystems coupled to specific antennae embedded in the thylakoid membrane, a common principle across diatoms, plants, and green algae. Still, unique featu...
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Article
Open AccessInfluence of the compatible solute sucrose on thylakoid membrane organization and violaxanthin de-epoxidation
The compatible solute sucrose reduces the efficiency of the enzymatic de-epoxidation of violaxanthin, probably by a direct effect on the protein parts of violaxanthin de-epoxidase which protrud...
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Article
Open AccessPre-purification of diatom pigment protein complexes provides insight into the heterogeneity of FCP complexes
Although our knowledge about diatom photosynthesis has made huge progress over the last years, many aspects about their photosynthetic apparatus are still enigmatic. According to published data, the spatial or...
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Article
15N photo-CIDNP MAS NMR on both photosystems and magnetic field-dependent 13C photo-CIDNP MAS NMR in photosystem II of the diatom Phaeodactylum tricornutum
Diatoms contribute about 20–25% to the global marine productivity and are successful autotrophic players in all aquatic ecosystems, which raises the question whether this performance is caused by differences i...
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Article
Open AccessAn optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transport
The preparation of functional thylakoid membranes from diatoms with a silica cell wall is still a largely unsolved challenge. Therefore, an optimized protocol for the isolation of oxygen evolving thylakoid mem...
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Article
Direct isolation of a functional violaxanthin cycle domain from thylakoid membranes of higher plants
A special domain of the thylakoid membrane of higher plants has been isolated which carries out the de-epoxidation of the xanthophyll cycle pigment violaxanthin to zeaxanthin.
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Chapter
Role of MGDG and Non-bilayer Lipid Phases in the Structure and Dynamics of Chloroplast Thylakoid Membranes
In this chapter we focus our attention on the enigmatic structural and functional roles of the major, non-bilayer lipid monogalactosyl-diacylglycerol (MGDG) in the thylakoid membrane. We give an overview on th...
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Article
Unusual features of the high light acclimation of Chromera velia
In the present study, the high light (HL) acclimation of Chromera velia (Chromerida) was studied. HL-grown cells exhibited an increased cell volume and dry weight compared to cells grown at medium light (ML). The...
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Article
Influence of thylakoid membrane lipids on the structure and function of the plant photosystem II core complex
MGDG leads to a dimerization of isolated, monomeric PSII core complexes. SQDG and PG induce a detachment of CP43 from the PSII core, thereby disturbing the intrinsic PSII electron transport. ...
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Article
Influence of pH, Mg2+, and lipid composition on the aggregation state of the diatom FCP in comparison to the LHCII of vascular plants
In the present study, the influence of Mg2+ ions and low pH values on the aggregation state of the diatom FCP and the LHCII of vascular plants was studied. In addition, the concentration of thylakoid membrane lip...
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Chapter
The Peculiar Features of Non-Photochemical Fluorescence Quenching in Diatoms and Brown Algae
Diatoms and brown algae are major contributors to marine primary production. They are biologically diverse, with thousands of different species, and are extremely successful, occupying almost every marine ecos...
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Chapter and Conference Paper
Effect of Lipids on Violaxanthin and Diadinoxanthin De-Epoxidation
Violaxanthin (Vx) and diadinoxanthin (Ddx) de-epoxidation are light dependent steps in one of the most important photoprotecting processes called respectively violaxanthin and diadinoxanthin cycle. Violaxanthi...
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Article
Molecular dynamics of the diatom thylakoid membrane under different light conditions
During the last years significant progress was achieved in unraveling molecular characteristics of the thylakoid membrane of different diatoms. With the present review it is intended to summarize the current k...
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Article
Regulation and function of xanthophyll cycle-dependent photoprotection in algae
The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and...
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Chapter
Lipids in Algae, Lichens and Mosses
Lipids from algae, lichens and mosses are highly diverse and differ from prokaryotic cyanobacteria and vascular plants in many aspects. Although in lower eukaryotes most of the lipids have functions similar to...
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Article
The synthesis of NPQ-effective zeaxanthin depends on the presence of a transmembrane proton gradient and a slightly basic stromal side of the thylakoid membrane
In the present study we address the question which factors during the synthesis of zeaxanthin determine its capacity to act as a non-photochemical quencher of chlorophyll fluorescence. Our results show that ze...
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Article
Structurally flexible macro-organization of the pigment–protein complexes of the diatom Phaeodactylum tricornutum
By means of circular dichroism (CD) spectroscopy, we have characterized the organization of the photosynthetic complexes of the diatom Phaeodactylum tricornutum at different levels of structural complexity: in in...
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Chapter and Conference Paper
The Oligomeric Antenna of the Diatom P. tricornutum — Localisation of Diadinoxanthin Cycle Pigments
In the present work a thorough examination of the oligomeric antenna of P. tricornutum was performed. Solubilisation of thylakoids with n-dodecyl β-d-maltoside in combination with gelfiltration and sucrose densit...
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Chapter and Conference Paper
Evidence for a Fast, Xanthophyll Cycle Independent NPQ Mechanism in the Diatom C. meneghiniana
for the diatom Phaeodactylum tricornutum it was reported that non-photochemical quenching (NPQ) strictly depends on the de-epoxidation state of the xanthophyll cycle (XC) pigment pool. In our present study we sho...
