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Article
Open AccessLineage recording in human cerebral organoids
Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of cell states within these systems; howe...
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Article
Open AccessActivation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors
DNA methylation contributes to the maintenance of genomic integrity in somatic cells, in part through the silencing of transposable elements. In this study, we use CRISPR-Cas9 technology to delete DNMT1, the DNA ...
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Protocol
Identifying miRNA Targets Using AGO-RIPseq
microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear t...
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Article
Open AccessDistinct cognitive effects and underlying transcriptome changes upon inhibition of individual miRNAs in hippocampal neurons
MicroRNAs (miRNA) are small, non-coding RNAs mediating post-transcriptional regulation of gene expression. miRNAs have recently been implicated in hippocampus-dependent functions such as learning and memory, a...
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Article
Open AccessIdentification of the miRNA targetome in hippocampal neurons using RIP-seq
MicroRNAs (miRNAs) are key players in the regulation of neuronal processes by targeting a large network of target messenger RNAs (mRNAs). However, the identity and function of mRNAs targeted by miRNAs in speci...
