3 Result(s)
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Charlotte Gustafsson
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Article
Open AccessT-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells
RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging.
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Article
Open AccessActivation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors
DNA methylation contributes to the maintenance of genomic integrity in somatic cells, in part through the silencing of transposable elements. In this study, we use CRISPR-Cas9 technology to delete DNMT1, the DNA ...
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Article
Open AccessHigh-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.
