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Article
Characterization of an African swine fever virus outbreak in India and comparative analysis of immune genes in infected and surviving crossbreed vs. indigenous Doom pigs
Since 2020, African swine fever (ASF) has affected all pig breeds in Northeast India except Doom pigs, a unique indigenous breed from Assam and the closest relatives of Indian wild pigs. ASF outbreaks result i...
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Article
Transcriptome signatures of host tissue infected with African swine fever virus reveal differential expression of associated oncogenes
African swine fever (ASF) has emerged as a threat to swine production worldwide. Evasion of host immunity by ASF virus (ASFV) is well understood. However, the role of ASFV in triggering oncogenesis is still un...
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Chapter
Host-Directed Antiviral Therapy
Most of the antiviral drugs developed so far have been specifically designed to target particular pathogens and their unique viral pathways. However, due to their pathogen-specific nature, these drugs may not ...
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Protocol
Lateral Flow Assay for Diagnosis of Pig Viral Diseases
Several infectious pig viral diseases of economic importance such as foot and mouth disease, Hog cholera, and African swine fever virus (ASFV) pose a serious threat to the swine industry worldwide. Control of ...
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Protocol
Production of Virus-like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis
Over many years, the baculovirus expression vector system (BEVS) has grown in popularity as a platform for expressing recombinant proteins. In addition to high levels of expression, the recombinant protein is ...
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Protocol
Multiplex PCR for Diagnosis of Porcine Diseases
Molecular diagnostics have revolutionized the efficiency of diagnosis of infectious diseases. An accurate diagnosis of a disease would prevent further infestation of infections in the healthy population. Altho...
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Protocol
Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases
Recombinant DNA technology or genetic engineering is one of the most explored technologies in the current era as it is one of the convincing technologies for the detection of both antigen and antibody. Recombi...
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Article
Development of a tetra-primer ARMS PCR-based assay for detection of a novel single-nucleotide polymorphism in the 5′ untranslated region of the bovine ITGB6 receptor gene associated with foot-and-mouth disease susceptibility in cattle
ITGB6 is known to be one of the major receptor components involved in host tropism of foot-and-mouth disease (FMD) virus in cattle. A competitive PCR technique called ARMS PCR was adapted to identify a single-...