5 Result(s)
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Article
Direct enzymatic sequencing of 5-methylcytosine at single-base resolution
5-methylcytosine (5mC) is the most important DNA modification in mammalian genomes. The ideal method for 5mC localization would be both nondestructive of DNA and direct, without requiring inference based on de...
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Article
Controllable genome editing with split-engineered base editors
DNA deaminase enzymes play key roles in immunity and have recently been harnessed for their biotechnological applications. In base editors (BEs), the combination of DNA deaminase mutator activity with CRISPR–C...
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Article
Mutations along a TET2 active site scaffold stall oxidation at 5-hydroxymethylcytosine
Saturation mutagenesis, molecular modeling and biochemical analysis revealed that active site interactions involving Thr1372 of TET2 are responsible for controlling its proficiency for stepwise oxidation of 5-...
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Article
AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation
AID/APOBEC deaminases, which convert cytosine bases to uracils in DNA and RNA, have recently been assigned a role in epigenetic regulation as components of DNA demethylation pathways. A systematic study shows ...
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Article
Grand Challenge Commentary: The chemistry of a dynamic genome
In the postsequencing era, chemical biology is uniquely situated to investigate genomic DNA alterations arising through epigenetic modifications, genetic rearrangements or active mutation. These transformation...
