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Article
Human lung cancer harbors spatially organized stem-immunity hubs associated with response to immunotherapy
The organization of immune cells in human tumors is not well understood. Immunogenic tumors harbor spatially localized multicellular ‘immunity hubs’ defined by expression of the T cell-attracting chemokines CXCL1...
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Article
Open AccessNucleotide imbalance decouples cell growth from cell proliferation
Nucleotide metabolism supports RNA synthesis and DNA replication to enable cell growth and division. Nucleotide depletion can inhibit cell growth and proliferation, but how cells sense and respond to changes i...
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Article
Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase
A bisulfite-free sequencing method yields high-confidence 5hmC profiles in low-input samples.
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Article
AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation
AID/APOBEC deaminases, which convert cytosine bases to uracils in DNA and RNA, have recently been assigned a role in epigenetic regulation as components of DNA demethylation pathways. A systematic study shows ...
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Article
Open AccessA tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples
Eukaryotic microorganisms are important but understudied components of the human microbiome. Here we present a pipeline for analysis of deep sequencing data on single cell eukaryotes. We designed a new 18S rRN...
