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Article
Open AccessRatiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation
Most CRISPR-based biosensors rely on labeled reporter molecules and expensive equipment for signal readout. A recent approach quantifies analyte concentration by sizing λ DNA reporters via gel electrophoresis,...
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Article
Adeno-associated virus genome quantification with amplification-free CRISPR-Cas12a
Efficient manufacturing of recombinant Adeno-Associated Viral (rAAV) vectors to meet rising clinical demand remains a major hurdle. One of the most significant challenges is the generation of large amounts of ...
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Article
Open AccessPrecise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis
Molecular diagnostics for crop diseases can guide the precise application of pesticides, thereby reducing pesticide usage while improving crop yield, but tools are lacking. Here, we report an in-field molecula...
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Article
Open AccessIntegrating charge mobility, stability and stretchability within conjugated polymer films for stretchable multifunctional sensors
Conjugated polymers (CPs) are promising semiconductors for intrinsically stretchable electronic devices. Ideally, such CPs should exhibit high charge mobility, excellent stability, and high stretchability. How...
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Protocol
Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens
Plant diseases pose a significant threat to global food security. Molecular diagnosis currently plays a crucial role in mitigating the negative impacts of plant diseases by accurately identifying the disease-c...
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Protocol
Smartphone Enabled Point-of-Care Detection of Serum Biomarkers
Sandwich immunoassays are the gold standard for detection of protein analytes. Here, we describe an ultrasensitive point-of-care sandwich immunoassay platform for the detection of biomarkers directly from bloo...
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Article
Open AccessAuthor Correction: Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates
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Article
Open AccessCellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates
Management of breast cancer in limited-resource settings is hindered by a lack of low-cost, logistically sustainable approaches toward molecular and cellular diagnostic pathology services that are needed to gu...
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Article
Open AccessAddressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to ...
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Article
Non-invasive plant disease diagnostics enabled by smartphone-based fingerprinting of leaf volatiles
Plant pathogen detection conventionally relies on molecular technology that is complicated, time-consuming and constrained to centralized laboratories. We developed a cost-effective smartphone-based volatile o...
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Article
Plasmonic molecular assays: Recent advances and applications for mobile health
Plasmonics-based biosensing assays have been extensively employed for biomedical applications. Significant advancements in use of plasmonic assays for the construction of point-of-care (POC) diagnostic methods...
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Article
Open AccessPlasmonics Enhanced Smartphone Fluorescence Microscopy
Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses...
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Article
Open AccessTargeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagn...
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Article
Open AccessOn-Chip Cytometry using Plasmonic Nanoparticle Enhanced Lensfree Holography
Computational microscopy tools, in particular lensfree on-chip imaging, provide a large field-of-view along with a long depth-of-field, which makes it feasible to rapidly analyze large volumes of specimen usin...
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Protocol
Gold Nanorods: Multifunctional Agents for Cancer Imaging and Therapy
Gold nanorods (GNRs) are strongly absorbing at near-infrared (NIR) frequencies and can be employed as multifunctional agents for biological imaging and theragnostics. GNRs can support nonlinear optical microsc...
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Article
Open AccessTwo-photon luminescence imaging of Bacillus spores using peptide-functionalized gold nanorods
Bacillus subtilis spores (a simulant of Bacillus anthracis) have been imaged by two-photon luminescence (TPL) microscopy, using gold nanorods (GNRs) functionalized with a cysteine-terminated homing peptide. Contr...
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Article
Open AccessThermosensitive Nanocables Prepared by Surface-Initiated Atom Transfer Radical Polymerization
Thermosensitive nanocables consisting of Au nanowire cores and poly(N-isopropylacrylamide) sheaths (denoted as Au/PNIPAAm) were synthesized by surface-initiated atom transfer radical polymerization (SI-ATRP). The...
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Article
The Escherichia coli O157:H7 DNA detection on a gold nanoparticle-enhanced piezoelectric biosensor
This paper presents development of a quartz crystal microbalance (QCM) biosensor for real-time detection of E. coli O157:H7 DNA based on nanogold particles amplification. Many inner Au nanoparticles were immobili...
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Article
Norvancomycin-capped silver nanoparticles: Synthesis and antibacterial activities against E. coli
The synthesis of norvancomycin (NVan)-capped silver nanoparticles (Ag@NVan) and their notable in vitro antibacterial activities against E. coli, a Gram-negative bacterial strain (GNB), are reported here. Mercapto...
