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Chapter
Drug-Resistant Epithelial Ovarian Cancer: Current and Future Perspectives
Epithelial ovarian cancer (EOC) is a complex disease with diverse histological subtypes, which, based on the aggressiveness and course of disease progression, have recently been broadly grouped into type I (lo...
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Article
Open AccessMolecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance
Alterations in key kinases and signaling pathways can fine-tune autophagic flux to promote the development of chemoresistance. Despite empirical evidences of strong association between enhanced autophagic flux...
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Article
Plasticity in Ovarian Cancer: The Molecular Underpinnings and Phenotypic Heterogeneity
Cellular plasticity, by large, is the ability through which cells morph into new phenotypic identity by trading-off with the previous one. This phenomenon has been observed in the normal and tumor cells in a v...
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Article
Selectivity Enhancement of Paclitaxel Liposome Towards Folate Receptor-Positive Tumor Cells by Ligand Number Optimization Approach
The present work aims to develop folate-targeted paclitaxel liposome (F-PTX-LIP), which will selectively target tumor cells overexpressing folate receptor (FR) and leave normal cells. Liposomes were prepared b...
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Protocol
Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function
Molecular functional imaging with optical reporter genes (both bioluminescence and fluorescence) is a rapidly evolving method that allows noninvasive, sensitive, real-time monitoring of many cellular events in...
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Protocol
Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging
Dual modality reporter genes are powerful means of tracking cellular processes in cell culture systems and whole animals. In this chapter, we describe the methods for construction of a plasmid reporter gene ve...
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Article
Open AccessCisplatin triggers cancer stem cell enrichment in platinum-resistant cells through NF-κB-TNFα-PIK3CA loop
Parallel to complex alteration in molecular and cellular events, enrichment of cancer stem cells (CSC) contributes significantly in deliberation and maintenance of cisplatin resistance. Cisplatin mediated CSC ...
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Article
Tumor suppressor protein p53 exerts negative transcriptional regulation on human sodium iodide symporter gene expression in breast cancer
Aberrant expression of human sodium iodide symporter (NIS) in breast cancer (BC) is well documented but the transcription factors (TF) regulating its aberrant expression is poorly known. We identify the presen...
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Article
Open AccessAn active IGF-1R-AKT signaling imparts functional heterogeneity in ovarian CSC population
Deregulated IGF-1R-AKT signaling influences multiple nodes of cancer cell physiology and assists in migration, metastasis and acquirement of radio/chemoresistance. Enrichment of cancer stem cells (CSC) positiv...
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Chapter
Stem Cells: The Holy Grail of Regenerative Medicine
Recent advancement in understanding the complex characters and interdependent molecular pathways of biological diseases led to a new therapeutic approach of “Biological Solutions to Biological Diseases.” Stem ...
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Article
Multimodality molecular imaging of disease progression in living subjects
The enormous advances in our understanding of the progression of diseases at the molecular level have been supplemented by the new field of ‘molecular imaging’, which provides for in vivo visualization of molecul...
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Chapter
Illustrating Molecular Events with Light: A Perspective on Optical Reporter Genes
A major medical success achieved in the last century was the decline of the overall mortality rate of cancer patients by providing high-quality medical care. Many groundbreaking discoveries along with imaging ...
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Article
Semiautomated Radiosynthesis and Biological Evaluation of [18F]FEAU: A Novel PET Imaging Agent for HSV1-tk/sr39tk Reporter Gene Expression
2′-Deoxy-2′-[18F]fluoro-5-ethyl-1-β-d-arabinofuranosyluracil ([18F]FEAU) is a promising radiolabeled nucleoside designed to monitor Herpes Simplex Virus Type 1 thymidine kinase (HSV1-tk) reporter gene expression ...
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Protocol
Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects
Bioluminescence imaging has become a very popular tool for noninvasive monitoring of fundamental biological and molecular processes in small living subjects. Luciferases are light-emitting enzymes that can gen...
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Article
A bubaline-derived satellite DNA probe uncovers generic affinities of gaur with other bovids
DNA ty** using genome derived cloned probes may be conducted for ascertaining genetic affinities of closely related species. We analysed gaurBos gaurus, cattleBos indicus, buffaloBubalus bubalis, sheepOvis arie...
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Article
Interaction of the non-protein-coding developmental and stress-induciblehsrω gene withRas genes ofDrosophila melanogaster
The93D orhsrω (hsr-omega) is an unusual non-protein-coding gene which shows a dynamic developmental expression in most cell types ofDrosophila melanogaster and which, besides being a member of the heat shock gene...
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Article
hsp 83 mutation is a dominant enhancer of lethality associated with absence of the non-protein codinghsrω locus inDrosophila melanogaster
Thehrsω or the 93D heat shock locus ofDrosophila melanogaster, which does not code for any protein, has an important role in development since nullosomy of this locus in transheterozygotes for two overlap** def...
