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Study ofO-sialylation of glycoproteins in C6 glioma cells treated with retinoic acid
When treated with retinoic acidin vivo, C6 glioma cells show an enhancement of CMP-Neu5Ac:Galβ 1–3 GalNAc-R α-2,3 sialyltransferase activity. A 300kDa glycoprotein was detected by lectin affinoblotting in retinoi...
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Article
Different reactivity of two Brain sialyltransferases towards sulfhydryl reagents. Evidence for a thiol group involved in the nucleotide-sugar binding site of the NeuAcα2-3Galβ1-3GalNAc α(2–6)sialyltransferase
We have studied the amino-acid residues involved in the catalytic activity of two distinct brain sialyltransferases acting on fetuin and asialofetuin. These two enzymes were strongly inhibited byN-bromosuccinimid...