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Article
Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer
Identifying metabolites and delineating their immune-regulatory contribution in the tumor microenvironment is an area of intense study. Interrogating metabolites and metabolic networks among immune cell subset...
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Article
Open AccessThe different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer
Protein glycosylation, the attachment of carbohydrates onto proteins, is a fundamental process that alters the biological activity of proteins. Changes to glycosylation states are associated with many forms of...
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Article
Open AccessCharacteristics and outcome of the COEUR Canadian validation cohort for ovarian cancer biomarkers
Ovarian carcinoma is the most lethal gynecological malignancy due to early dissemination and acquired resistance to platinum-based chemotherapy. Reliable markers that are independent and complementary to clini...
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Article
Open AccessTumor-infiltrating lymphocytes predict response to anthracycline-based chemotherapy in estrogen receptor-negative breast cancer
Infiltration of breast tumors by tumor-infiltrating lymphocytes (TIL) has been associated with sensitivity to anthracycline-based chemotherapy. However, it is unclear whether this is true within the estrogen r...
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Article
Estrogen Receptor Alpha Phosphorylated at Tyrosine 537 is Associated with Poor Clinical Outcome in Breast Cancer Patients Treated with Tamoxifen
A phospho-tyrosine 537-ERα (p-Y537-ERα) antibody was validated for immunohistochemistry of formalin-fixed paraffin-embedded human breast cancer biopsies and used to interrogate multiple ER positive breast canc...
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Article
Density of tumour stroma is correlated to outcome after adoptive transfer of CD4+ and CD8+ T cells in a murine mammary carcinoma model
Adoptive immunotherapy shows promise for the treatment of cancer; however, partial or mixed responses remain common outcomes due to the heterogeneity of tumours. We studied three murine mammary tumour lines th...
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Article
Immunohistochemical validation of multiple phospho-specific epitopes for estrogen receptor α (ERα) in tissue microarrays of ERα positive human breast carcinomas
Estrogen receptor α (ERα) activity is regulated by phosphorylation at several sites. Recently several antibodies specific for individual phosphorylated sites within ERα have became available. Such antibodies p...
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Article
Open AccessEvolutionary concepts in biobanking - the BC BioLibrary
Medical research to improve health care faces a major problem in the relatively limited availability of adequately annotated and collected biospecimens. This limitation is creating a growing gap between the pa...
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Article
Mammary tumors with diverse immunological phenotypes show differing sensitivity to adoptively transferred CD8+ T cells lacking the Cbl-b gene
We tested the efficacy of CD8+ T cells lacking the Cbl-b gene against a panel of mammary tumor lines with different intrinsic sensitivities to T cells. Mice bearing established tumors expressing an ovalbumin-tagg...
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Article
Open AccessSteroid Receptor RNA Activator Protein (SRAP): a potential new prognostic marker for estrogen receptor-positive/node-negative/younger breast cancer patients
The steroid receptor RNA activator is a functional RNA suspected to participate in the mechanisms underlying breast tumor progression. This RNA is also able to encode for a protein, Steroid Receptor RNA Activa...
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Article
Claudins 1, 3, and 4 protein expression in ER negative breast cancer correlates with markers of the basal phenotype
In the present study we investigated the protein expression of claudins 1, 3, and 4 and their relationship to clinical variables and outcome in a cohort of ER−ve and ER+ve human invasive breast cancers. Immuno...
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Article
Open AccessJab1 is a target of EGFR signaling in ERα-negative breast cancer
c-Jun activation domain-binding protein-1 (Jab1) is a multifunctional signaling protein that previously has been shown to be a master regulator of a poor prognostic gene signature in invasive breast cancer and...
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Article
Estrogen receptor-β regulates psoriasin (S100A7) in human breast cancer
We have previously observed a paradoxical relationship of the psoriasin/S100A7 gene with estrogen response in-vitro in ERα positive cells but its association with ERα negative status in-vivo raising the possib...
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Article
Assessment of multiple different estrogen receptor-β antibodies for their ability to immunoprecipitate under chromatin immunoprecipitation conditions
Several different antibodies to total estrogen receptor (ER)β, ERβ1 and ERβ2/cx have been tested and compared for their ability to immunoprecipitate ERβ specific isoforms under chromatin immunoprecipitation co...
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Article
Open AccessReal-time PCR complements immunohistochemistry in the determination of HER-2/neu status in breast cancer
The clinical benefit of determining the status of HER-2/neu amplification in breast cancer patients is well accepted. Although immunohistochemistry (IHC) is the most frequently used method to assess the over-expr...
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Protocol
Breast Tissue Banking
A “tumor bank” provides a resource tool that can efficiently address the limitations of other tissue resources, such as clinical archives and research study collections. A tumor bank allows formal accreditatio...
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Article
Open AccessExpression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis
The human psoriasin (S100A7) gene has been implicated in inflammation and tumor progression. Implementation of a mouse model would facilitate further investigation of its function, however little is known of t...
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Article
S100A7 and the progression of breast cancer
The S100 gene family comprises more than 20 members whose protein sequences encompass at least one EF-hand Ca2+ binding motif. The expression of individual family members is not ubiquitous for all tissues and the...
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Article
S100A7 (psoriasin) expression is associated with aggressive features and alteration of Jab1 in ductal carcinoma in situof the breast
The S100A7 (psoriasin) gene is highly expressed in ductal carcinoma in situ (DCIS) of the breast and can be downregulated in invasive carcinoma. Persistent S100A7 expression in invasive carcinoma is associated wi...
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Article
Mammographic density is related to stroma and stromal proteoglycan expression
Mammographic density and certain histological changes in breast tissues are both risk factors for breast cancer. However, the relationship between these factors remains uncertain. Previous studies have focused...
