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  1. No Access

    Article

    Holographic Study of the Response of HeLa Cells to Photodynamic Treatment with Endogenously Generated Protoporphyrin IX

    Changes in morphological and optical parameters of HeLa cells preincubated with 5-aminolevulinic acid (5-ALA), after photodynamic treatment (PDTr) with different intensity of excitation radiation are studied b...

    A. A. Zhikhoreva, A. V. Belashov, A. A. Akhundzyanov… in Optics and Spectroscopy (2022)

  2. No Access

    Article

    Linear Dichroism and Birefringence of Probe Radiation in Pump-Probe Spectroscopy of Polyatomic Molecules

    The dichroism and birefringence effects in excited states of polyatomic molecules upon excitation by two successive femtosecond pulses have been studied theoretically as a function of the delay time between th...

    B. V. Semak, O. S. Vasyutinskii in Optics and Spectroscopy (2021)

  3. No Access

    Article

    Dynamics of 5-Aminolevulinic Acid-Induced Accumulation of Protoporphyrin IX in Three Cell Lines of Different Origin

    The dynamics of synthesis and accumulation of protoporphyrin IX induced by 5-aminolevulinic acid (5-ALA) was studied using confocal fluorescence microscopy in three established cell lines: immortalized cells o...

    D. A. Gorbenko, A. V. Belashov, T. N. Belyaeva, E. S. Kornilova… in Technical Physics (2021)

  4. No Access

    Article

    Decay Times and Anisotropy in Polarized Fluorescence of Flavin Adenine Dinucleotide Determined with Subnanosecond Resolution

    Optical properties of biological coenzyme FAD in an aqueous solution have been studied. Two fluorescence lifetimes, a rotational diffusion time, and an anisotropy parameter have been determined from experiment...

    M. K. Krasnopevtseva, V. P. Belik, I. V. Semenova… in Technical Physics Letters (2020)

  5. No Access

    Article

    Observation of Anisotropic Relaxation in Biological Molecules with Subpicosecond Temporal Resolution

    A new pump–probe technique has been developed for investigation of anisotropic relaxation and energy transfer processes in excited states of polyatomic molecules excited by femtosecond laser pulses. The propos...

    I. A. Gorbunova, M. E. Sasin, O. S. Vasyutinskii in Technical Physics Letters (2020)

  6. No Access

    Article

    The Development of Segmentation Algorithms in Holographic Microscopy and Tomography for Determination of Morphological Parameters of Cells

    Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The p...

    A. V. Belashov, D. A. Gorbenko, A. A. Zhikhoreva… in Technical Physics Letters (2019)

  7. No Access

    Article

    Phosphorescence Kinetics of Singlet Oxygen in HeLa Cells Suspension

    The kinetics of singlet oxygen phosphorescence in HeLa cells suspended in phosphate buffered saline (PBS) being generated under photosensitized excitation with Radachlorin photosensitizer has been observed and...

    V. P. Belik, D. M. Beltukova, T. N. Belyaeva, I. M. Gadzhiev… in Technical Physics Letters (2019)

  8. No Access

    Article

    Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

    The decay of polarized fluorescence in coenzyme NADH in aqueous-methanol solution under two-photon excitation by femtosecond laser pulses within the spectral range of 720–780 nm has been studied. Molecular flu...

    M. E. Sasin, I. A. Gorbunova, N. O. Bezverkhnii… in Technical Physics Letters (2019)

  9. No Access

    Article

    Morphological Changes in Malignant Tumor Cells at Photodynamic Treatment Assessed by Digital Holographic Microscopy

    The paper presents an investigation of cell death dynamics and changes of cellular morphology induced by the intracellular generation of singlet oxygen as a result of photodynamic treatment. The response of ki...

    A. A. Zhikhoreva, A. V. Belashov, D. A. Gorbenko… in Russian Journal of Physical Chemistry B (2019)

  10. No Access

    Article

    Polarized Fluorescence in Tryptophan Excited by Two-Photon Femtosecond Laser Pulses

    The decay of polarized fluorescence of tryptophan in water-propylene glycol solution two-photon excited by femtosecond laser pulses was explored. Linearly and circularly polarized light in the wavelength band ...

    M. E. Sasin, A. G. Smolin, O. S. Vasyutinskii in Optics and Spectroscopy (2018)

  11. No Access

    Article

    Spectral Characteristics of the DMEM Cell-Culture Medium

    Spectral characteristics of luminescence of the DMEM cell-culture medium are studied. The luminescence spectrum of the medium is determined in the visible and near-IR spectral ranges at wavelengths of up to 13...

    A. A. Zhikhoreva, V. P. Belik, A. V. Belashov, O. S. Vasyutinskii… in Technical Physics (2018)

  12. No Access

    Article

    Polarized Fluorescence in Indole under Two-Photon Excitation by Femtosecond Laser Pulses

    We have analyzed the decay of fluorescence intensity in indole dissolved in propylene glycol under two-photon excitation by linearly and circularly polarized femtosecond laser pulses in the wavelength range 47...

    M. E. Sasin, V. I. Tushkanov, A. G. Smolin, P. S. Shternin… in Technical Physics (2018)

  13. No Access

    Article

    Luminescence of Radachlorin Photosensitizer in Aqueous Solution under Excitation at 405 and 660 nm

    Entire luminescence spectrum of a commercial photosensitizer Radachlorin in aqueous solution has been recorded under laser excitation at 660 nm and analyzed. The peak of singlet oxygen phosphorescence at 1274 ...

    D. M. Beltukova, V. P. Belik, O. S. Vasyutinskii, I. M. Gadzhiev… in Optics and Spectroscopy (2018)

  14. No Access

    Article

    Determination of the refractive index of dehydrated cells by means of digital holographic microscopy

    Spatial distributions of the integral refractive index in dehydrated cells of human oral cavity epithelium are obtained by means of digital holographic microscopy, and mean refractive index of the cells is det...

    A. V. Belashov, A. A. Zhikhoreva, V. G. Bespalov… in Technical Physics Letters (2017)

  15. No Access

    Article

    Fluorescence anisotropy of indole molecules under two-photon excitation in the spectral range of 485–510 nm

    Decay of polarized fluorescence in indole dissolved in propylene glycol under two-photon excitation by femtosecond laser pulses in the wavelength range of 485–510 nm has been studied. It is shown that under th...

    M. E. Sasin, V. I. Tushkanov, A. G. Smolin, O. S. Vasyutinskii in Optics and Spectroscopy (2017)

  16. No Access

    Article

    Study of the relaxation rate of photoexcited indole molecules by the interferometric pump-and-probe method at picosecond resolution

    We present a new interferometric method that can be used for studying the dynamics of photoinduced processes in biologically important molecules at ultrahigh temporal resolution. The method is based upon the d...

    A. L. Glazov, A. D. Il’ina, A. A. Sukharev, O. S. Vasyutinskii in Technical Physics Letters (2017)

  17. No Access

    Article

    Polarized fluorescence in NADH under two-photon excitation with femtosecond laser pulses

    Polarized fluorescence decay in NADH molecules in aqueous solution under two-photon excitation by femtosecond laser pulses has been studied. The excitation was carried out by linear and circularly polarized ra...

    O. S. Vasyutinskii, A. G. Smolin, C. Oswald, K. H. Gericke in Optics and Spectroscopy (2017)

  18. No Access

    Article

    Photobleaching mechanisms of Radachlorin photosensitizer in aqueous solution

    The time dependence of the fluorescence intensity of aqueous solutions of Radachlorin photosensitizer at different concentrations and the influence of the dissolved oxygen concentration on the fluorescence dyn...

    D. M. Beltukova, O. S. Vasyutinskii, A. L. Glazov… in Optics and Spectroscopy (2017)

  19. No Access

    Article

    Crystallization of amorphous hydrogenated silicon (a-Si:H) films under irradiation with femtosecond laser pulses

    Crystallization of thin films of amorphous hydrogenated silicon under the irradiation of femtosecond laser pulses has been studied. It was found that the crystallization has a clearly pronounced threshold natu...

    V. P. Belik, O. S. Vasyutinskii, A. V. Kukin, M. A. Petrov… in Technical Physics Letters (2016)

  20. No Access

    Article

    The influence of excitation radiation parameters on photosensitized generation of singlet oxygen in water

    Photosensitized generation of singlet oxygen with the aid of Radahlorin® photosensitizer has been investigated. The dependences of the intensity of singlet oxygen phosphorescence and photosensitizer fluorescen...

    A. D. Il’ina, A. L. Glazov, I. V. Semenova, O. S. Vasyutinskii in Optics and Spectroscopy (2016)

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