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    Protocol

    Analysis of Calcium-Induced Conformational Changes in Calcium-Binding Allergens and Quantitative Determination of Their IgE Binding Properties

    The polcalcin family is one of the most epidemiologically relevant families of calcium-binding allergens. Polcalcins are potent plant allergens that contain one or several EF-hand motifs and their allergenicit...

    Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso… in Calcium-Binding Proteins and RAGE (2013)

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    Article

    A Modified Protocol for RNA Isolation from High Polysaccharide Containing Cupressus arizonica Pollen. Applications for RT–PCR and Phage Display Library Construction

    RNA isolation is the first step in the study of gene expression and recombinant protein production. However, the isolation of high quantity and high-quality RNA from tissues containing large amounts of polysac...

    Yago Pico de Coaña, Nuria Parody, Enrique Fernández-Caldas… in Molecular Biotechnology (2010)