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Article
Open AccessProgrammable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease
Highly infectious illnesses caused by pathogens constitute severe threats to public health and lead to global economic loss. The use of robust and programmable clustered regularly interspaced short palindromic...
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Article
Open AccessHACE1-mediated NRF2 activation causes enhanced malignant phenotypes and decreased radiosensitivity of glioma cells
HACE1, an E3 ubiquitin-protein ligase, is frequently inactivated and has been evidenced as a putative tumor suppressor in different types of cancer. However, its role in glioma remains elusive. Here, we observ...
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Article
Open AccessSynergistic activation of mutant TERT promoter by Sp1 and GABPA in BRAFV600E-driven human cancers
The activating TERT promoter mutations and BRAFV600E mutation are well-established oncogenic alterations in human cancers. Coexistence of BRAFV600E and TERT promoter mutations is frequently found in multiple canc...
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Article
Open AccessCYP2S1 is a synthetic lethal target in BRAFV600E-driven thyroid cancers
BRAFV600E is the most common genetic alteration and has become a major therapeutic target in thyroid cancers; however, intrinsic feedback mechanism limited clinical use of BRAFV600E specific inhibitors. Synthetic...
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Article
Open AccessProgress in exosome associated tumor markers and their detection methods
Exosomes are secreted by cells and are widely present in body fluids. Exosomes contain various molecular constituents of their cells of origin such as proteins, mRNA, miRNAs, DNA, lipid and glycans which are v...
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Article
Open AccessApplication of magnetic nanoparticles in nucleic acid detection
Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infecti...
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Article
A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids
A variety of automated sample-in-answer-out systems for in vitro molecular diagnostics have been presented and even commercialized. Although efficient in operation, they are incapable of quantifying targets, s...
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Article
The development of a rapid high-quality universal nucleic acid extraction kit based on magnetic separation
Nucleic acid extraction is one of the bases in molecular biology and it is the indispensable process for nucleic acid detection. Due to its excellent characteristics, the method based on magnetic beads has bec...
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Article
Open AccessAptamer selection and applications for breast cancer diagnostics and therapy
Aptamers are short non-coding, single-stranded oligonucleotides (RNA or DNA) developed through Systematic Evolution of Ligands by Exponential enrichment (SELEX) in vitro. Similar to antibodies, aptamers can bi...
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Article
The aptamers generated from HepG2 cells
Liver cancer, as the second cause of cancer death all around the world, resulted in a series of chronic liver diseases. More than 80% of the patients cannot receive effective treatment because of their advance...
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Article
Open AccessInjectable hydrogels for cartilage and bone tissue engineering
Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potenti...
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Article
Synthesis of aptamer-functionalized Ag nanoclusters for MCF-7 breast cancer cells imaging
Aptamer-functionalized silver nanoclusters (Ag NCs) have been attracting a lot of interest as label-free probes which have been successfully applied to both cell imaging and molecular detection. MUC1 aptamer i...
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Article
Progress on sensors based on nanomaterials for rapid detection of heavy metal ions
The heavy metal ions, especially Cd2+, Pb2+ and Hg2+, show extremely hazard to the environment and human being. The measurement of heavy metal ions using sensors is catching more and more attention for its advant...
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Article
Open AccessLong Blood Residence and Large Tumor Uptake of Ruthenium Sulfide Nanoclusters for Highly Efficient Cancer Photothermal Therapy
Transition metal sulfide (TMS) holds great potential in cancer photothermal therapy (PTT) because of the high absorbance in the near-infrared (NIR) region. The short blood circulation time and limited tumor ac...
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Article
A new method for improving the accuracy of miRNA detection with NaYF4:Yb,Er upconversion nanoparticles
MicroRNAs (miRNAs) are small noncoding RNAs, which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis. So far, man...
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Applications of Magnetic Nanoparticle-Based High-Throughput Single-Nucleotide Polymorphism Genoty** Platforms in Gastric Cancer Research
To realize SNP genoty** of gastric cancer can direct reasonable choice of therapeutic drugs of gastric cancer. This chapter summarizes magnetic nanoparticles-based high-throughput single-nucleotide polymorph...
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Article
Open AccessRecent advances in nano scaffolds for bone repair
Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials (that is, DNA). In bone tissue, na...
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Article
Open AccessFluorescence based Aptasensors for the determination of hepatitis B virus e antigen
This research is aimed at selecting specific aptamer of hepatitis B e antigen by SELEX and its applications. Hepatitis B e antigen (HBeAg) seroconversion is used as an indicator of virological response when tr...
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Article
Ultrasensitive quantitation of MicroRNAs via magnetic beads-based chemiluminesent assay
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level, and their aberrant expression occurs during the development of malignant diseases. Recently, miRNAs...
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Article
Open AccessA Novel Electrochemical Microfluidic Chip Combined with Multiple Biomarkers for Early Diagnosis of Gastric Cancer
Early diagnosis is very important to improve the survival rate of patients with gastric cancer and to understand the biology of cancer. In order to meet the clinical demands for early diagnosis of gastric canc...
