-
Article
Efficient mesophyll-derived protoplast manipulation system as a versatile tool for characterization of genes responding to multiple stimuli in mulberry
Mulberry serves as a crucial element of the global sericulture industry. Functional gene analysis considering resistant traits improvement of mulberry is greatly hindered by its long life cycle and recalcitran...
-
Article
Open AccessChromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization
Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequ...
-
Article
Open AccessLepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins
Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resi...
-
Article
Open AccessAmplification of miniature inverted-repeat transposable elements and the associated impact on gene regulation and alternative splicing in mulberry (Morus notabilis)
Miniature inverted-repeat transposable elements (MITEs) are common in eukaryotic genomes, and are important for genomic evolution.
-
Article
Open AccessThe molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition
Chilling is common in nature and can damage most plant species, particularly young leaves and buds. Mulberry (Morus spp.) is an economically important food source for the domesticated silkworm (Bombyx mori). Howe...
-
Article
LysM1 in MmLYK2 is a motif required for the interaction of MmLYP1 and MmLYK2 in the chitin signaling
Two LysM-containing proteins, namely, MmLYP1 and MmLYK2, were identified in mulberry. These proteins might be involved in chitin signaling. The LysM1 of MmLYK2 is critical for their interactions.
-
Article
Open AccessIdentification of Morus notabilis MADS-box genes and elucidation of the roles of MnMADS33 during endodormancy
The MADS-box genes encode transcriptional regulators with various functions especially during floral development. A total of 54 putative Morus notabilis MADS-box genes (MnMADSs) were identified and phylogenetical...
-
Article
Open AccessFISH-based mitotic and meiotic diakinesis karyotypes of Morus notabilis reveal a chromosomal fusion-fission cycle between mitotic and meiotic phases
Mulberry (Morus spp.), in family Moraceae, is a plant with important economic value. Many polyploid levels of mulberry have been determined. In the present study, the fluorescence in situ hybridization (FISH) tec...
-
Article
Anatomical and transcriptional dynamics of early floral development of mulberry (Morus alba)
Sex determination of flowers is a critical component of reproductive biology in plants. The plant hormone ethylene promotes female flower formation in mulberry. Illumina RNA-Seq technology was used for de novo...
-
Article
Open AccessEvolutionary and functional analysis of mulberry type III polyketide synthases
Type III polyketide synthases are important for the biosynthesis of flavonoids and various plant polyphenols. Mulberry plants have abundant polyphenols, but very little is known about the mulberry type III pol...
-
Article
Biotic stress-induced expression of mulberry cystatins and identification of cystatin exhibiting stability to silkworm gut proteinases
Biotic stresses induce the expression of mulberry cystatins. MaCPI-4 protein is stable in silkworm digestive fluid and accumulates in gut food debris and frass.
-
Article
Open AccessNonfunctional ingestion of plant miRNAs in silkworm revealed by digital droplet PCR and transcriptome analysis
Since a plant miRNA (miR168) cross-regulating a mammalian transcript was reported, miRNA-mediated cross-kingdom communication has become one of the most compelling but controversial topics. In the present stud...
-
Article
Open AccessIdentification of the mulberry genes involved in ethylene biosynthesis and signaling pathways and the expression of MaERF-B2-1 and MaERF-B2-2 in the response to flooding stress
The phytohormone ethylene is essential to plant growth and development. It plays crucial roles in responses to biotic and abiotic stress. The mulberry tree is an important crop plant in countries in which peop...
-
Article
Cloning and expression analyses of the anthocyanin biosynthetic genes in mulberry plants
Anthocyanins are natural food colorants produced by plants that play important roles in their growth and development. Mulberry fruits are rich in anthocyanins, which are the most important active components of...
-
Article
Open AccessDraft genome sequence of the mulberry tree Morus notabilis
Human utilization of the mulberry–silkworm interaction started at least 5,000 years ago and greatly influenced world history through the Silk Road. Complementing the silkworm genome sequence, here we describe ...
-
Article
Open AccessComparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation
Microsporidian Nosema bombycis has received much attention because the pébrine disease of domesticated silkworms results in great economic losses in the silkworm industry. So far, no effective treatment could be ...
-
Article
Open AccessMicroarray-based gene expression profiles of silkworm brains
Molecular genetic studies of Bombyx mori have led to profound advances in our understanding of the regulation of development. Bombyx mori brain, as a main endocrine organ, plays important regulatory roles in vari...
-
Article
Molecular cloning and expression analysis of Bmrbp1, the Bombyx mori homologue of the Drosophila gene rbp1
RBP1 is an important splicing factor involved in alternative splicing of the pre-mRNA of Drosophila sex-determining gene dsx. In this work, the Bombyx mori homologue of the rbp1 gene, Bmrbp1, was cloned. The pre-...
-
Article
Open AccessExpression profile of cuticular genes of silkworm, Bombyx mori
Insect cuticle plays essential roles in many physiological functions. During molting and metamorphosis tremendous changes occur in silkworm cuticle where multiple proteins exist and genes encoding them constit...
-
Article
Sequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori)
A defensin-like gene, BmdefA, was rediscovered in the silkworm genome and expressed sequence tags databases. The open reading frame of BmdefA encodes a prepropeptide consisting of a 22-residue signal peptide, a 3...
