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Article
Open AccessThe performance of homopolymer detection using dichromatic and tetrachromatic fluorogenic next-generation sequencing platforms
Homopolymer (HP) sequencing is error-prone in next-generation sequencing (NGS) assays, and may induce false insertion/deletions and substitutions. This study aimed to evaluate the performance of dichromatic an...
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Article
Open AccessCharacterization and mitigation of artifacts derived from NGS library preparation due to structure-specific sequences in the human genome
Hybridization capture-based targeted next generation sequencing (NGS) is gaining importance in routine cancer clinical practice. DNA library preparation is a fundamental step to produce high-quality sequencing...
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Article
Open AccessHelicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer
Helicobacter pylori, particularly cytotoxin-associated gene A (CagA)-positive strains, plays a key role in the progression of gastric cancer (GC). Ferroptosis, associated with lethal lipid peroxidation, has emerg...
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Enhancing Students’ Field Experience in Physical Geography Courses Using Virtual Reality Technology
This chapter outlines how Virtual Reality (VR) technologies were used to enhance the field experience in undergraduate physical geography courses in a Hong Kong university. First, we examine the reason for inc...
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Article
Open AccessChromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization
Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequ...
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Article
Open AccessTargeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer
KRAS is an important tumor intrinsic factor driving immune suppression in colorectal cancer (CRC). In this study, we demonstrate that SLC25A22 underlies mutant KRAS-induced immune suppression in CRC. In immuno...
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Article
Transcriptional analysis of the dimorphic fungus Umbilicaria muehlenbergii reveals the molecular mechanism of phenotypic transition
The lichen-forming fungus Umbilicaria muehlenbergii undergoes a phenotypic transition from a yeast-like to a pseudohyphal form. However, it remains unknown if a common mechanism is involved in the phenotypic swit...
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Article
Disentangling Diet- and Medium-Associated Microbes in Sha** Daphnia Gut Microbiome
Host genotype and environment are considered crucial factors in sha** Daphnia gut microbiome composition. Among the environmental factors, diet is an important factor that regulates Daphnia microbiome. Most of ...
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Article
Open AccessThe cholesterol uptake regulator PCSK9 promotes and is a therapeutic target in APC/KRAS-mutant colorectal cancer
Therapeutic targeting of KRAS-mutant colorectal cancer (CRC) is an unmet need. Here, we show that Proprotein Convertase Subtilisin/Kexin type 9 (PSCK9) promotes APC/KRAS-mutant CRC and is a therapeutic target. Us...
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Article
SEAM is a spatial single nuclear metabolomics method for dissecting tissue microenvironment
Spatial metabolomics can reveal intercellular heterogeneity and tissue organization. Here we report on the spatial single nuclear metabolomics (SEAM) method, a flexible platform combining high-spatial-resoluti...
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Article
Open AccessAnti-oncogene PTPN13 inactivation by hepatitis B virus X protein counteracts IGF2BP1 to promote hepatocellular carcinoma progression
Hepatitis B x protein (HBx) affects cellular protein expression and participates in the tumorigenesis and progression of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Metabolic reprogramming ...
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Article
Open AccessApatinib Monotherapy for Chemotherapy-Refractory Metastatic Colorectal Cancer: A Multi-centre, Single-Arm, Prospective Study
Angiogenesis inhibitors are of considerable interest for treating metastatic colorectal cancer (mCRC). This trial evaluated the efficacy and safety of apatinib in chemotherapy-refractory mCRC. Apatinib 500 mg ...
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Article
Water masses influence bacterioplankton community structure in summer Kongsfjorden
To ascertain the saying “Everything is everywhere, but the environment selects”, it was imperative to find out the main factor influencing bacterioplankton composition at genus level of Kongsfjorden where was ...
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Article
Open AccessThe double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer
As a key glycolysis enzyme, the significance of pyruvate dehydrogenase kinase 1 (PDK1) in the development of colorectal cancer (CRC) remains unknown. This study revealed that the prognosis of CRC patients with...
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Article
Magnesium depletion suppresses the anti-grazer colony formation in Scenedesmus obliquus
In aquatic ecosystems, many phytoplankton species have evolved various inducible defense mechanisms against the predation. The expression of these defenses is affected by environmental conditions such as nutri...
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Article
Spatiotemporal characteristics of seasonal precipitation and their relationships with ENSO in Central Asia during 1901–2013
The vulnerable ecosystem of the arid and semiarid region in Central Asia is sensitive to precipitation variations. Long-term changes of the seasonal precipitation can reveal the evolution rules of the precipit...
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Analyzing the Uncertainties of Ground Validation for Remote Sensing Land Cover Map** in the Era of Big Geographic Data
Ground validation and accuracy assessment on remote sensing land cover classification is a vital process before the product may be used by the end-user. The common approach towards the validation is based on f...
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Article
Open AccessDistribution patterns of haplotypes for symbionts from Umbilicaria esculenta and U. muehlenbergii reflect the importance of reproductive strategy in sha** population genetic structure
The diversity of lichen fungal components and their photosynthetic partners reflects both ecological and evolutionary factors. In present study, molecular investigations of the internal transcribed spacer of t...
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Article
Open AccessComparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms
The lichen-forming fungus was isolated from the desert lichen Endocarpon pusillum that is extremely drought resistant. To understand the molecular mechanisms of drought resistance in the fungus, we employed RNA-s...
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Article
Open AccessThe inhibitory effects of extracellular ATP on the growth of nasopharyngeal carcinoma cells via P2Y2 receptor and osteopontin
Nasopharyngeal carcinoma (NPC) is a common malignant tumor observed in the populations of southern China and Southeast Asia. However, little is known about the effects of purinergic signal on the behavior of N...
