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Article
Open AccessA pairwise cytokine code explains the organism-wide response to sepsis
Sepsis is a systemic response to infection with life-threatening consequences. Our understanding of the molecular and cellular impact of sepsis across organs remains rudimentary. Here, we characterize the path...
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Article
Open AccessBioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons
Progress in biological imaging is intrinsically linked to advances in labeling methods. The explosion in the development of high-resolution and super-resolution imaging calls for new approaches to label target...
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Article
A whole-tissue RNA-seq toolkit for organism-wide studies of gene expression with PME-seq
The immune system operates at the scale of the whole organism in mammals. We currently lack experimental approaches to systematically track and study organism-wide molecular processes in mice. Here we describe...
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Article
Suppression by TFR cells leads to durable and selective inhibition of B cell effector function
Follicular regulatory T cells (TFR cells) inhibit follicular helper T cell (TFH cell)-mediated antibody production. Sharpe and colleagues show that TFR cells induce a distinct suppressive state in TFH cells and B...
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Article
Deciphering molecular circuits from genetic variation underlying transcriptional responsiveness to stimuli
A computational pipeline identifies genetic variants that affect transcriptional responses to stimuli, and uses this information to refine molecular circuits.
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Article
Detection of pathogenic intestinal bacteria by Toll-like receptor 5 on intestinal CD11c+ lamina propria cells
Toll-like receptors (TLRs) recognize distinct microbial components and induce innate immune responses. TLR5 is triggered by bacterial flagellin. Here we generated Tlr5−/− 1mice and assessed TLR5 function in vivo....
