12 Result(s)
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Nicola J. Stonehouse
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Article
Open AccessPublisher Correction: A conserved glutathione binding site in poliovirus is a target for antivirals and vaccine stabilisation
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Article
Open AccessA conserved glutathione binding site in poliovirus is a target for antivirals and vaccine stabilisation
Strategies to prevent the recurrence of poliovirus (PV) after eradication may utilise non-infectious, recombinant virus-like particle (VLP) vaccines. Despite clear advantages over inactivated or attenuated vir...
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Article
Open AccessHigher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for genome replication
Replication of many positive-sense RNA viruses occurs within intracellular membrane-associated compartments. These are thought to provide a favourable environment for replication to occur, concentrating essent...
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Article
Open AccessStructural insight into Pichia pastoris fatty acid synthase
Type I fatty acid synthases (FASs) are critical metabolic enzymes which are common targets for bioengineering in the production of biofuels and other products. Serendipitously, we identified FAS as a contamina...
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Article
An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells
Enteroviruses comprise a large group of mammalian pathogens that includes poliovirus. Pathology in humans ranges from sub-clinical to acute flaccid paralysis, myocarditis and meningitis. Until now, all of the ...
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Protocol
Recombinant Expression of Tandem-HBc Virus-Like Particles (VLPs)
The hepatitis B virus (HBV) core protein (HBc) has formed the building block for virus-like particle (VLP) production for more than 30 years. The ease of production of the protein, the robust ability of the co...
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Article
Open AccessProduction and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates
The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus ...
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Article
Open AccessEvaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research
Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. A...
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Article
Open AccessYIP1 family member 4 (YIPF4) is a novel cellular binding partner of the papillomavirus E5 proteins
E5 proteins are amongst the least understood of the Human Papillomavirus (HPV) encoded gene products. They are small, membrane-integrated proteins known to modulate a number of critical host pathways associate...
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Chapter
Virus Assembly and Morphogenesis
This chapter will deal with the assembly of macromolecular complexes, in particular the assembly and dynamics of simple viruses. At first glance this may seem an unusual topic for inclusion in a discussion of ...
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Article
Crystal structures of a series of RNA aptamers complexed to the same protein target
We have determined the crystal structures, at 2.8 Å resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the...
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Article
Crystal structure of an RNA bacteriophage coat protein–operator complex
THE RNA bacteriophage MS2 is a convenient model system for the study of protein–RNA interactions. The MS2 coat protein achieves control of two distinct processes—sequence-specific RNA encapsidation and repress...
