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  1. Article

    Open Access

    Modified Northern blot protocol for easy detection of mRNAs in total RNA using radiolabeled probes

    Northern blotting is still used as a gold standard for validation of the data obtained from high-throughput whole transcriptome-based methods. However, its disadvantages of lower sensitivity, labor-intensive o...

    Tao Yang, Mingdi Zhang, Nianhui Zhang in BMC Genomics (2022)

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  2. No Access

    Article

    Altered Localization of the δ Subunit of the GABAA Receptor in the Thalamus of α4 Subunit Knockout Mice

    The α4 subunit of the GABAA receptor (GABAAR) is highly expressed in the thalamus where receptors containing the α4 and δ subunits are major mediators of tonic inhibition. The α4 subunit also exhibits considerabl...

    Zechun Peng, Nianhui Zhang, Dave Chandra, Gregg E. Homanics… in Neurochemical Research (2014)

  3. No Access

    Article

    Sensitivity of Prestaining RNA with Ethidium Bromide Before Electrophoresis and Performance of Subsequent Northern Blots Using Heterologous DNA Probes

    Adding ethidium bromide (EtBr) at low concentrations to RNA samples before running formaldehyde–agarose gels affords the advantages of checking RNA integrity and evaluating the quality of size-separation at an...

    Yun Zhao, Linfang Du, Nianhui Zhang in Molecular Biotechnology (2013)

  4. No Access

    Article

    Isolation of RNA of high quality and yield from Ginkgo biloba leaves

    An improved protocol was developed to isolate total RNA in good yield and integrity from Ginkgo biloba leaves containing high levels of flavonoid glycosides, terpene lactones, carbohydrates and polyphenolic secon...

    Tao Wang, Nianhui Zhang, Lingfang Du in Biotechnology Letters (2005)

  5. No Access

    Article

    Distribution of glutamate-like and glutamine-like immunoreactivities in the rat organ of Corti: a light microscopic and semiquantitative electron microscopic analysis with a note on the localization of aspartate

    The light- and electron microscopic localization of glutamate and glutamine in the rat organ of Corti was studied by means of antisera raised against the respective amino acids coupled to carrier proteins. The...

    Shin-ichi Usami, Kirsten K. Osen, Nianhui Zhang… in Experimental Brain Research (1992)

  6. No Access

    Article

    Distribution of glutamine-like immunoreactivity in the cerebellum of rat and baboon (Papio anubis) with reference to the issue of metabolic compartmentation

    The cellular and subcellular localization of glutamine, a major glutamate precursor, was studied by means of an antiserum raised against glutaraldehydefixed glutamine. Ultrathin sections from the cerebellar co...

    Nianhui Zhang, Jon Laake, Erlend Nagelhus, Jon Storm-Mathisen… in Anatomy and Embryology (1991)